Case Study for Cherry Temp

Test of Cherry Temp

Case study: The 1st division of C. elegans embryo thanks to precise temperature shifts

Courtesy of  Dr. Gotta and Dr. Meraldi (Gotta and Meraldi labs)

check C. elegans pack

Experimental conditions

Strain was cultured at 22°C using standard methods (Brenner, 1974). The strain used was TH65 (YFP::α-tubulin; Schlaitz et al., 2007).

For live imaging, embryos were cut from gravid hermaphrodites in egg buffer (118 mM NaCl, 48 mM KCl, 2 mM CaCl2, 2 mM MgCl2, and 25 mM Hepes, pH 7.5) and mounted on 2% noble agar pads.

Divisions were recorded using a microscope (DM6000; Leica) equipped with epifluorescence and DIC optics and a camera (DFC 360 FX; Leica). Images were collected every 15 sec using a 63×/1.4 NA objective and LAS AF software (Leica). Embryos were imaged at 22°C and the temperature was shifted to 5°C at 0sec thanks to the special control stage equipment. After 195 sec at 5°C,  the temperature was shifted back to 22°C. Temperature control and tempertaure shifts were performed using Cherry Temp.

1st division of C. elegans embryo

COULD IT WORK FOR ME?

Your Email (required)

Your Message

captcha

Observation

At 5°C = depolymerization of the microtubules (in green), less fluorescence observed.

As soon as the temperature goes back to 22°C, the microtubule polymerize again and the fluorescence intensity increased

Rapid event (we were able to observe the changes within few seconds)

c

c

c

Results

Images representative of timelapse images of the 1st division of C. elegans embryo movie

COULD IT WORK FOR ME?

Your Email (required)

Your Message

captcha

Cherry Temp

CherryTemp,  

Robuste and user-friendly

Temperature range for your sample: from 15°C to 25°C

Precision:  at 0.1°C

Delay for temperature shifts: 10 seconds

Types of microscope:  any microscopes

CherryCare service available for Microscope facility

COULD IT WORK FOR ME?

Your Email (required)

Your Message

captcha

    This is a unique website which will require a more modern browser to work! Please upgrade today!