Frequently Asked Questions

Can I test your system?

We usually don’t offer this possibility as we have too much demand and we cannot satisfy it. If your application fits in one of our product packs, feel free to get in touch and we will be happy to give you more details. Of course we can also put you in contact with some of our customers or partners who can tell you more about the system from a user point of view.

What is the delivery time?

Our delivery time is currently 4 months after receipt of a valid Purchase Order. However, for urgent reasons (such as grant particularities, financial exercise closure…), do not hesitate to get in touch and we will work to find a solution.

What is the price of CherryTemp?

It depends on what you need (a full platform, customized or not, the number of microscopes you want to use it on, or if you want an OEM solution). Get in touch and one of our experts will answer you  within 24 hours to understand your need and give you more details.

Are you installing the system in my lab?

We do not need to come to install the system as we set-up a dedicated installation process. We first  interact with you before the shipping to understand your set up and constraints so that we can provide a platform adapted to your environment and tips for an easy installation. We then send the system and we accompany your team with remote sessions to make sure all the users will get all the knowledge for a smooth utilization of the system.

Our priority is to make things simple for you so we ensure full after-sales service and we will always be available by email/skype to assist you and answer any question.

How intrusive is the platform around the microscope? Is it plug and play and easy to remove in case of shared microscopes?

We have worked hard to make the system as small and non intrusive as possible so that it can stay around the microscope even when you don’t use it. It will not interfere with other equipment. It is also easy to plug and unplug. After the first 30 minutes installation, it only takes 10 minutes to start your experimentation.

The system consists in several elements which allow customized and flexible installation around the microscope.

  • Two boxes (CherryTemp [Lxlxh = 270*190*170mm] and CherryLoop [210*210*70mm]) can be installed on a shelf at 0 to 2m far from the microscope.
  • One tank (standard 500ml lab bottle) containing the thermalization liquid.
  • The heat exchangers (40*80*20mm) placed nearby the observation area on a provided holder which does not interfere with the experimentation and does not weigh top stages/piezzo systems.
  • The microfluidic chip which is the same length and width than your coverslide and 7mm thick is positioned on a provided and dedicated chip holder compatible with the standard stage insert of inverted microscopes.

On which microscopes CherryTemp can be installed?

CherryTemp fits any  microscopes (upright and inverted). We developed dedicated ergonomic elements to fit any brands of microscope (Zeiss AxioImager, Nikon Ti Eclipse, Olympus IX81…).

How can you be sure that the sample is at the right temperature?

This is the central strength of CherryTemp. We have developed an embedded calibration that is totally transparent from the user point-of-view. Indeed, our software integrates a dedicated calibration which takes into account the heat sink played by the objective and constantly monitors the room temperature. In addition, we check every system with an infra-red thermal camera to ensure that every manufactured system answers our quality standards. Altogether, we ensure a 0.1°C precision, and a 0.5°C stability over time on the 5 to 45°C range.

What is the accuracy of the temperature?

It is a strong point of our CherryTemp system to ensure temperature precision but also homogeneity and stability over time. The temperature is 0.5°C homogenous on the sample area, precision is 0.1°C and stability over time is guaranteed to remain under 0.5°C.

This is excellent specifications compared to most systems where there can be several degrees difference and big homogeneity problems, which can become worse with the heat sink of immersion objectives or the extra heating of halogen lamps.

Which calibration methods do you use?

We use a combination of several methods for extra accuracy, combining a thermal camera to validate all manufactured systems, microelectrodes (see 4-points electrode deposit method), numerical algorithms and finally biological confirmation experiments as a last check.

If you are interested in this specific topic, get in touch and we will happy to give you in depth insights on these methods.

Is CherryTemp compatible with both air and immersion objective lenses?

Yes, it is. We performed several experiments and set up a specific calibration. Our software integrates these data and calculates a correcting factor if an immersion objective is used for your experimentation. As a consequence, the temperature you set is exactly the one that is experienced by your cells.

How long is the shift between two temperatures?

Thanks to our dual-channel heat exchangers, a shift between two temperatures takes only a few seconds (10 seconds) and does not rely on the starting or ending setting: the highest is the gradient between two temperatures, the fastest is the speed of change therefore it takes around 10 seconds both for a 5°C delta change or a 40°C delta change.

Does the temperature shift generate mist on the lamella?

The system itself does not generate any mist and we have not had any problem with this in the experiment we conducted in house or with customers. Mist can appear when using air objective lenses. This is a physical parameter that no system can avoid but which is obviously removed whith immersion objective lenses.

The major source for mist would be a drop of room temperature if room air is very humid. In this case, mist would appear as the lamella cools down. However, microscopy rooms are often air conditioned or at least controlled to avoid such drops.

Is there any evaporation?

Evaporation of the media would occur at the same rate you are used to with a mounting system comprising coverslide and coverslip with no media renewal. We can work together to find solutions thanks to our microfluidic expertize to minimize or avoid evaporation issues.

How is your mounting system? I don’t really understand where is my sample?

Basically, your sample/cells are in sandwich between the microscope slide and a standard cover slip. We simply add on the top of this mounting system our microfluidic chip so that the cover slip that closes our chip will transmit its temperature to the sample by contact with the sample.

Can i reuse the chips?

Yes you can reuse the chips as much as you want, we can provide additionnal chips if required, get in touch with our services.

How thick is the cover slip?

You can use your ordinary 170µm slips. The provided microfluidic chip has a refractive index similar to the air one which ensures no interference with the lightpath of your microscope.

Can I use my own chip (meaning, is there a different temperature calibration)?

Yes each chip requires a specific calibration: this is why we propose standard chips that are adapted to your usage so that you can benefit from our calibration.

Is there a pressure generator in the system? Can I use my own flow rate system?

We offer several options adapted to your needs. In the context of the full plug and play CherryTemp platform, we provide a dedicated closed loop fluidic system with a pump regulating the liquid flow.

This all inclusive system offers several advantages :

  • It ensures that the sample temperature corresponds to the settings because our calibrations are performed with the constant flow rate provided by this pump/loop system.
  • It facilitates the setup as there is only one tank.

However, if you already have pumping equipment, just get in touch and we will assess compatibility of your needs and our system. As long as you respect the minimal flow rate conditions that we provide (at least 3.5ml/min), thermalization will be guaranteed.

What is the flow rate, does my biological sample is in direct contact?

In the standard CherryTemp platform, the fluid loop is for temperature control only and is not in direct contact with the biological sample so you don’t have to worry about the flow rate.

What should we do after an experimentation (purge fluidic system or not)?

The maintenance of the system is very simple, we provide a full user guide to help you manage the fluid system. We have a 5 minutes cleaning step at the end of every experimentation to keep the system protected from any biological contamination.

Is there an electronic control of the system? Is there a software provided?

Yes. We have a dedicated software (CherrySoft) which controls the entire system. We had a particular attention to make it simple and ergonomic with its small windows that can be arrange in parallel of your microscopy software. It just takes a click of a mouse to set up the temperature in the system and perform the ultr-fast temperature changes.

Can I use my own calibration table to use my customized corrections rather than yours?

Yes. We can provide a free-of-calibration software to let you controlling specific setups. However, if you use your own chips, you will have to make your own calibration.

What is the difference between the different CherryTemp Packs?

The only differences are linked to the mounting system for your samples. We optimized the observation area to fit your needs. The specifications are only guided by the requirements of your experimentations.The core CherryTemp system remains unchanged so that you have nearly no extra-costs if you plan to use different models.

Is the system compatible with 35mm Petri dishes or compartmented IBD chambers?

No. We choose to keep a standard “cover slip” design to fit as many applications as possible.

Do the microfluidic chip interfers with light ?

No it does not. The refraction index of all the system components (especially the chips) is equivalent to air so it does not interfere with the condenser light, DIC etc.

Is there any risk for losing the focus point when shifting from one temperature to another?

You are right, a change in temperature will create a focus drift. This is a physical law that we cannot change. However, your microscope might be equipped with an autofocus system. As this delta-focus variation is constant between your two temperatures the focus change is predictable and you can save the proper focus point before starting the experiment.

How efficient is your after-sale service?

Our customers are our main priority and we make sure we provide the best  after-sales service:

- answer by email, within 24h,

- live meeting with skype when it suits you

- free diagnosis by a product engineer

- keep cool, we remotely take control of your system

- Remote fixing  for  90% of the case

- free shipping  

 

What is Cherry Care?

Cherry Care is an additional service we designed, you can subsribe to the service at any time, there are options for 6months engagement to 1 year or even 3 years (Cherry care gold).

“Cherry care” offers:

  • Monthly automatic full check-up thanks to our metrology program  (anticipation of the ageing)
  • any hardware issues (considering a normal use of the system, according to the warrantee conditions) fixed for free
  • UPS shipping free included
  • 3 weeks engagement for replacement or we lend you a machine for free.
  • 2 skype/ phone call meetings with one of our technicien to answer questions, install updates, control machine
  • disposable products refills (Liquids + chips) every 6 months

If you are interested, get in touch with our services.

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