Yeast Pack: what can we observe when using Cherry Temp for yeasts?

We developped the fastest heater-cooler for microscopy. It enables ultra-fast temperature shifts [10 seconds] from any temperature to any other in the 5-45°C range. CherryTemp fits any microscope. You can now control in real-time your favorite molecular processes, observe dynamic events linked to temperature on live and discover unthought mechanisms.

Temperature shift to image S.pombe mutants

Application example: Block and release of CDC25 S. pombe ts mutants.

CDC25-22 h+ S. pombe mutants where first blocked for 4 hours at the restrictive 36.5°C temperature (Pict. A). Division was bloqued until temperature had been quickly changed (in 10 seconds) to the permissive 25°C. No septum could be observed  after 15 minutes of release (Pict. B, 0%) compared to a large 93% proportion of cells showing a septum after 90 minutes (Pict. C).

Observation was performed on a Zeiss Axio Observer Z1, fully motorized inverted microscope with an ASI PZ-2000FT XY-Z Piezo stage and Zeiss Definite Focus using a 63X oil objective (Zeiss Plan-Apochromat).

We thank Damien Coudreuse’s lab (Julien Babic, PhD st. – IGDR, Rennes) for credits.

CDC25-ts-S-pombe-block-and-release-by-CherryTemp

Temperature modification from 25°C to 37°C resulting in a reversible arrest of mitosis.

Application example: Mitosis arrest of yeast CDC25 ts mutants.

CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp

S. pombe thermosensitive mutants (CDC25 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of yeast samples between 25°C and 37°C resulting in a reversible arrest of mitosis.

Yeast cold-shock microtubules depolymerization with CherryTemp

We depolymerized S. pombe microtubules while imaging the cells (gamma-tubulin GFP) with a cold shock at 5°C (25°C to 5°C in less than 10 sec). We then repolymerized by shifting back the temperature within seconds to 25°C.

Released spindle elongation by quickly shifting the temperature back to 25°C (in less that 10 sec)

We controlled early mitotic spindle growth in cut7-ts S. pombe mutants while imaging the cells (gamma-tubulin GFP).
We blocked spindle elongation at restrictive temperature (36°C). Note that spindles, before anaphase (e.g. top-left and top-right corners) stay monopolar during 7 minutes.
We then released spindle elongation by quickly shifting the temperature back to 25°C (in less that 10 sec).

How long is it to install cherry temp? From yeasts to observation in 30 seconds

Change the temperature of your sample within seconds during live-cell imaging

Initially developed for PCR military application, the world fastest temperature controller for microscopy is now available for live-cell imaging.

Get benefit of our robust and precise temperature calibration. Our software continuously integrates changes of your room temperature. It also takes into account the heat sink played by immersion objective lenses.

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Cherry Temp, the faster heater cooler for yeast imaging


Yeast imaging & temperature

ULTRAFAST SHIFTS | 10 SECONDS

Shift in less than 10 seconds and observe restrictive temperatures on live

LARGE RANGE | 5 TO 45°C

Depolymerise microtubules | Play with ts mutants

YEAST DEDICATED SLIDE

Use a dedicated slide…stay focused!

FITS ANY MICROSCOPE

CherryTemp can be easily installed and shared between different microscopes. We have a set of ergonomic elements fitting main brands (Nikon, Zeiss, Olympus, Leica) for both upright and inverted scopes.

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