How to choose the right system for your sample?
Temperature control devices: a comparative guide to choose the suitable temperature control device for your experimental requirement:
Microfluidic temperature controller: CherryTemp
The technology relies on a sophisticated, electronically controlled microfluidics loop associated with ultra-fast heater-cooler exchangers system.
Δ Temperature: 5 to 45C
Pros: ultra-fast temperature shift (5 to 45 C in seconds), temperature stability (±0,1C),dual-temperature applied on sample, immersion objective and DIC illumination heat loss integrated correction, sub-cellular high-resolution imaging, no cell stress
Cons: not adequate for long-run experiments
Microscope stage temperature is controlled using Peltier or liquid based heated platform. Temperature controlled microscope stages modify the temperature of the biological sample by thermal diffusion through the glass slide or metal heat conducting bridge to minimize the temperature gradient over the sample.
ΔTemperature: -196°C to 100°C (for the best set-up)
Pros: high temperature range, fast temperature switch (2-3 min), easy to use
Cons: poor temperature uniformity, mono-temperature applied on sample, additional temperature control for immersion objective required, single area temperature control, heat-diffusion to nearby microscope equipment
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This type of devices provides a multi-parameters (gas, pH and temperature) controlled environment within a plexiglas box.
Δ Temperature: 15 to 60C
Pros: easy to use, homogenous temperature, fits Petri dishes, long run experiments
Cons: slow temperature switch, no temperature gradient, temperature loss during long DIC illumination or immersion objective, mono-temperature applied on sample, cumbersome if intervention is needed on the sample
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The dish is directly perfused with a pre-heated fluid. This system is adapted for experiments requiring rapid temperature shift with medium renewal
ΔTemperature : ambient to 50C
Pros: fast temperature switch (less than a minute), homogenous temperature, objective-induced heat loss is controlled by warm fluid, medium renewal
Cons: cell stress due to shear fluid flow forces, flushing of chemical molecules due to fluid flow, fluid movement affects focal plane, mono-temperature applied on sample
They are used as a complement to perfusion systems or thermalized stage in order to minimize the immersion objective thermal gradient. There exists electronic heater collars and fluidic collars.
ΔTemperature: ambient to 43C, if thermalized+pumping system 0 to 100C
Pros: enhance temperature uniformity of pre-existing temperature controller devices, large temperature range
Cons: in complement with other temperature controller, slow temperature shift, difficult of use