Temperature control for microscope imaging microscope stage heater cooler

[et_pb_section background_image=”http://www.cherrybiotech.com/wp-content/uploads/2014/03/soft-lithography-wafer-degrade1.jpg” background_color=”#ffffff” inner_shadow=”off” parallax=”off”][et_pb_row][et_pb_column type=”1_2″][et_pb_text admin_label=”Temperature control for microscopy imaging: microscope stage” background_layout=”light” text_orientation=”left”]

Temperature control for microscopy imaging: heater/cooler microscope stages

[/et_pb_text][/et_pb_column][et_pb_column type=”1_2″][et_pb_image admin_label=”Image microscope” src=”http://www.cherrybiotech.com/wp-content/uploads/2014/03/Microscope1-e1396630683458.png” url_new_window=”off” animation=”off” module_class=”tuto” /][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off”][et_pb_row][et_pb_column type=”1_2″][et_pb_text admin_label=”Introduction” background_layout=”light” text_orientation=”left” module_class=”tuto”]

Introduction

Biological processes are very sensitive, and respond rapidly to temperature variation. Performing live-cell imaging implies to maintain the cells temperature as long as image acquisition is required. Several temperature controllers are available on the market, all have advantages and caveats and depending on your experimental needs they may meet your needs. Microscope stage heater/cooler is one way to control or shift the temperature of a sample.

[/et_pb_text][et_pb_text admin_label=”How does it work?” background_layout=”light” text_orientation=”left”]

Precision-30x28 How does it work?

Microscope stages heater/cooler are a substitute to your regular stage. They have a very high thermal capacity and use resistive heaters or Peltier devices to heat or cool down the sample. Temperature controlled microscope stages change the temperature by thermal diffusion through the glass slide or metal heat conducting bridge, which reduce the formation of thermal gradient on the sample. Microscope stages temperature range from -20C to 100C and temperature-shift occurs within minutes.

[/et_pb_text][et_pb_text admin_label=”Advantages” background_layout=”light” text_orientation=”left”]

Precision-30x28 Advantages

 Large temperature range

-20C to 100C, the use of a Peltier element allows microscope stage to reach température below ambient. This is an important features, notably for scientists studying microtubules assembly or vesicular transport.

 Fast temperature switch

Since the surface to thermalize is reduced to the microscope stages, the speed of température Switch is much faster than for an incubation box. Depending on the power of the temperature actuators, the temperature switch can be achieved in 2 to 3 min, but overshoots of several °C should be expected when system speed is increased.

 Easy access to the cells

Depending on the choosen setting, manipulator can have access to his cells during his experiment. Possibility to add drugs or medium without changing the objective focus or stopping the recording of the ongoing experiment.

[/et_pb_text][/et_pb_column][et_pb_column type=”1_2″][et_pb_text admin_label=”disadvantages” background_layout=”light” text_orientation=”left” module_class=”tuto”]

Precision-30x28Disadvantages

Poor temperature uniformity across the sample

Microscope stages control sample temperature using thermal diffusion. The heat transfer from the temperature control device to the sample can generate thermal gradient up to several degree on the sample.

 Requires objective heater

The use of oil or water immersion objectives will require the use of an objective heater. Indeed, when the objective, the oil, and coverslip make contact, the objective acts as a heat sink leading to 5-7C in sample temperature (room with a view). Also, a strong gradient can arise if the microscope stage temperature shift response is faster or slower than with the collar system.

 Requires dehumidifier

While microscope stages allow to reach temperature below ambient, it is recommend to use a dehumidifier to prevent condensation.

 Lack of active cooling system

Since there is no active cooling system, samples are subjected to strong temperature increase when using DIC illumination or during extended observation under epi-fluorescence microscopy. There is also a heat diffusion to nearby microscope element which may affect the objective focus.

 Mono temperature control

Only one temperature can be set on the microscope stage temperature controller. While other systems allows to set two température at once and shift from one to the other ultra-rapidly.

[/et_pb_text][et_pb_text admin_label=”Text” background_layout=”light” text_orientation=”left”]

[button link=”http://www.cherrybiotech.com/?p=534″ color=”red”] Use CherryTemp for temperature control[/button][button link=”http://www.cherrybiotech.com/?p=1404″ color=”red”] Discover yeast applications with CherryTemp[/button] [button link=”http://www.cherrybiotech.com/?p=1428″ color=”red”] Discover C.elegans applications with CherryTemp[/button][button link=”http://www.cherrybiotech.com/?p=1503″ color=”red”] Discover any cell type applications with CherryTemp[/button]

[/et_pb_text][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off” background_color=”#f04848″ inner_shadow=”off” parallax=”off”][et_pb_row][et_pb_column type=”1_2″][et_pb_cta admin_label=”Call To Action How does CherryTemp™ work?” title=”How does CherryTemp™ work?” button_url=”http://www.cherrybiotech.com/?p=534″ button_text=”Understand how it works” background_color=”#f04848″ use_background_color=”on” background_layout=”dark” text_orientation=”left”]

CherryTemp reaches such performance thanks to breaking edge microfluidics.

And thanks to a lot of work from our team to make sure Cherry Temp integrates seemlessly in your microscope set up.

[/et_pb_cta][/et_pb_column][et_pb_column type=”1_2″][et_pb_divider admin_label=”Divider” color=”#ffffff” show_divider=”off” height=”10″ /][et_pb_slider admin_label=”Slider” show_arrows=”on” show_pagination=”on” auto=”off” parallax=”off”][et_pb_slide background_image=”http://www.cherrybiotech.com/wp-content/uploads/2014/04/CherryTemp-microfluidic-platform-for-microscopy-temperature-control-3.jpg” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][et_pb_slide background_image=”http://www.cherrybiotech.com/wp-content/uploads/2014/04/microfluidic-chip-for-live-cell-imaging.jpg” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][et_pb_slide background_image=”http://www.cherrybiotech.com/wp-content/uploads/2014/04/confocal-inverted-microscope.png” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][et_pb_slide background_image=”http://www.cherrybiotech.com/wp-content/uploads/2014/04/CherryTemp-and-CherryLoop-temperature-control-for-microscopy-2.jpg” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][/et_pb_slider][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off”][et_pb_row][et_pb_column type=”1_4″][et_pb_blurb admin_label=”Blurb E njoy unprecendeted performance” title=”Enjoy the performance” url=”http://www.cherrybiotech.com/?p=312″ url_new_window=”off” image=”http://www.cherrybiotech.com/wp-content/uploads/2014/03/icon_rook2.png” alt=”Enjoy unprecendeted performance” animation=”off” background_layout=”light” text_orientation=”center”]

CherryTemp is the fastest temperature control in the 5-45°C range
[button color=”silver” link=”http://www.cherrybiotech.com/?p=312″] Learn more[/button]

[/et_pb_blurb][/et_pb_column][et_pb_column type=”1_4″][et_pb_blurb admin_label=”Blurb Work with any type of live cell” title=”Work with any of live cell” url_new_window=”off” image=”http://www.cherrybiotech.com/wp-content/uploads/2014/03/icon_grid-3×32.png” alt=”Work with any type of live cell” animation=”off” background_layout=”light” text_orientation=”center” url=”http://www.cherrybiotech.com/?p=1346″]

CherryTemp is adapted to all types of live cells including S. pombe and C. elegans
[button color=”silver” link=”http://www.cherrybiotech.com/?p=1346″]Learn more[/button]

[/et_pb_blurb][/et_pb_column][et_pb_column type=”1_4″][et_pb_blurb admin_label=”Blurb Start experiments in minutes” title=”Start experiments in minutes” url_new_window=”off” image=”http://www.cherrybiotech.com/wp-content/uploads/2014/03/icon_hourglass2.png” animation=”off” background_layout=”light” text_orientation=”center” url=”http://www.cherrybiotech.com/?p=896″]

CherryTemp is easy to use so that you focus on your research, not the instrument
[button color=”silver” link=”http://www.cherrybiotech.com/?p=896″] Learn more[/button]

[/et_pb_blurb][/et_pb_column][et_pb_column type=”1_4″][et_pb_divider admin_label=”Divider” color=”#ffffff” show_divider=”off” height=”15″ /][et_pb_text admin_label=”Text C elegans” background_layout=”light” text_orientation=”center” module_class=”elegans”]

S. pombe

See another example of temperature control with thermosensitive mutants

[button color=”silver” link=”http://www.cherrybiotech.com/?p=2131″] Application note[/button]

[/et_pb_text][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off”][et_pb_row][et_pb_column type=”4_4″][et_pb_cta admin_label=”Call To Action” title=”Want to know more about the system?” button_url=”http://www.cherrybiotech.com/contact-us” button_text=”Contact us” background_color=”#a40000″ use_background_color=”on” background_layout=”dark” text_orientation=”center”]

contact@cherrybiotech.com

[/et_pb_cta][/et_pb_column][/et_pb_row][/et_pb_section]

This is a unique website which will require a more modern browser to work! Please upgrade today!

Social Media Auto Publish Powered By : XYZScripts.com