Temperature control for microscope imaging perfusion system

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Temperature control for microscope imaging: micro perfusion system

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Introduction

Using microscopy imaging to address cell biology questions requires maintaining the cells in a physiological-like environment. There are several temperature controllers available on the market; all have positive and negative features and depending on users needs some might be best suited than others. Micro-perfusion systems are commonly used to control sample temperature at the microscope stage.

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Precision-30x28How does it work?

Micro-perfusion systems are composed of a culture chamber connected to a perfusion system allowing fluid renewal in the chamber. There exist open or closed chambers. Open chamber allow a direct access to the cells, and are convenient for cells injection. Closed chambers prevent medium evaporation and better-controlled environmental parameters (gas, pH); they are more appropriate for long-term imaging. Micro perfusion systems provide fast temperature switch and the possibility to renew medium during the experiment. Those temperature control systems are  based on the fast injection of thermalized culture medium directly on the sample. The “in-flow” thermalization of the cell culture medium requires a good management of the heat exchange system to prevent strong deviations between temperature control settings and sample real temperature.

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Precision-30x28Advantages

Speed of the temperature switch (~1 min)

Since temperature controlled micro perfusion systems directly thermalize the sample by changing the cell culture medium, temperature switch can be achieved in less than 1 min when extreme flows are generated around the sample.

Efficient thermal transfer

Forced liquid convection is efficient for thermal transfer. Hence, temperature-controlled  micro perfusion systems provide a good temperature uniformity across the sample. Temperature controlled  micro perfusion systems also reduce temperature increase when turning on DIC illumination or during extended observations with epi-fluorescence.

 Medium renewal, drug injection

Temperature controlled  micro perfusion systems are based on medium renewal with the possibility to inject drugs. In this system the cells are kept in a physiological-like environment. They are particularly suited for long-term imaging.

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Precision-30x28Disadvantages

High shear stress

Because temperature-controlled  micro perfusion systems generate strong flow rate on the cells, they  induce high shear stress on them. Moreover, because of fluid flow-rate chemical signaling molecules can be washed out too quickly from cells environment. Finally, cells in suspension, loosely adherent cells or very sensitive cells such as stem cells might be simply washed out or suffer to much mechanical stress from the flow-rate.

Mono temperature control

Only one temperature can be set on the microscope stage temperature controller. While other systems allows to set two temperatures at once and shift from one to the other ultra-rapidly

Requires an objective collar

Using immersion objectives will require additional temperature controlled objectives collar to reduce the temperature gradient between observation zone and the rest of the biological sample.

Ambient and above

Most of the micro perfusion systems work on a temperature range  from ambiant to 50°C, some of them propose also a cooling system to reach 0C but they require a specific additional cooling microscope stage adapter.

Requires experimented users

Temperature-controlled micro-perfusion systems are best for experimented users as it requires a strong knowledge to make the system works correctly.

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[button link=”http://www.cherrybiotech.com/temperature-control-for-microscopy” color=”red”] Use CherryTemp for temperature control[/button][button link=”http://www.cherrybiotech.com/temperature-control-for-yeast-microscopy” color=”red”] Discover yeast applications with CherryTemp[/button] [button link=”http://www.cherrybiotech.com/temperature-control-for-c-elegans-worms-microscopy” color=”red”] Discover C.elegans applications with CherryTemp[/button][button link=”http://www.cherrybiotech.com/temperature-controller-for-live-cell-biology-imaging” color=”red”] Discover any cell type applications with CherryTemp[/button]

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CherryTemp reaches such performance thanks to breaking edge microfluidics.

And thanks to a lot of work from our team to make sure Cherry Temp integrates seemlessly in your microscope set up.

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CherryTemp is the fastest temperature control in the 5-45°C range
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CherryTemp is adapted to all types of live cells including S. pombe and C. elegans
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CherryTemp is easy to use so that you focus on your research, not the instrument
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S. pombe

See another example of temperature control with thermosensitive mutants

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contact@cherrybiotech.com

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