S. pombe application note

[et_pb_section background_image=”https://www.cherrybiotech.com/wp-content/uploads/2014/03/soft-lithography-wafer-degrade1.jpg” background_color=”#ffffff” inner_shadow=”off” parallax=”off”][et_pb_row][et_pb_column type=”1_2″][et_pb_text admin_label=”Controlling S. pombe mitosis with the latest CherryTemp™ platform” background_layout=”light” text_orientation=”left”]

S. pombe

mitosis control

with thermosensitive mutants

Astuce

An example using CherryTemp™ for fast and accurate temperature switch during microscopic observation.

[/et_pb_text][/et_pb_column][et_pb_column type=”1_2″][et_pb_image admin_label=”Image_S. pombe grey” src=”https://www.cherrybiotech.com/wp-content/uploads/2014/04/S.-pombe-fission-yeast-temperature-control-for-live-cell-imaging.png” url_new_window=”off” animation=”off” module_class=”tuto” /][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off”][et_pb_row][et_pb_column type=”4_4″][et_pb_cta admin_label=”Call To Action” button_url=”https://www.cherrybiotech.com/?p=1404″ button_text=”Find it useful? Learn more about it!” use_background_color=”off” background_layout=”dark” text_orientation=”center” background_color=”#ffffff” title=”Call to action – find it useful?” /][/et_pb_column][/et_pb_row][et_pb_row][et_pb_column type=”1_2″][et_pb_text admin_label=”Abstract/Introduction” background_layout=”light” text_orientation=”left” module_class=”tuto”]

Context

CDC25 protein dynamic control of microtubules

Fig.1: CDC25 protein is fonctional at 25°C (a) but becomes inactivated at 37°C (b)

During S. pombe division, the CDC25 protein is required for mitosis completion. At 25°C the CDC25 protein is functional (Fig. 1a) but over 37°C it becomes non-functional or inactivated (Fig. 1b). Such a mutant is called “thermosensitive” and as mitosis only occurs at 25°C, this temperature is termed “permissive”. In these experiments, we used the CherryTemp precise temperature control, yet hitherto impossible to achieve, to impact S. pombe physiology during cell division.

[/et_pb_text][/et_pb_column][et_pb_column type=”1_2″][et_pb_text admin_label=”Results” background_layout=”light” text_orientation=”left” module_class=”tuto”]

Results

During our experimentation, S. pombe thermosensitive mutants (CDC25 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of yeast samples between 25°C and 37°C resulting in a reversible arrest of mitosis.

CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp

Fig 2: CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp
Yeast division occurs normally at 25°C (a) but is suddenly stopped when temperature is quickly shifted to 37°C (b). Mitosis only restarts when temperature is set back to 25°C (c, d, e, f)

[/et_pb_text][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off” background_color=”#f04848″ inner_shadow=”off” parallax=”off”][et_pb_row][et_pb_column type=”1_2″][et_pb_cta admin_label=”Call To Action How does CherryTemp™ work?” title=”How does CherryTemp™ work?” button_url=”https://www.cherrybiotech.com/?p=534″ button_text=”Understand how it works” background_color=”#f04848″ use_background_color=”on” background_layout=”dark” text_orientation=”left”]

CherryTemp reaches such performance thanks to breaking edge microfluidics.

And thanks to a lot of work from our team to make sure Cherry Temp integrates seemlessly in your microscope set up.

[/et_pb_cta][/et_pb_column][et_pb_column type=”1_2″][et_pb_divider admin_label=”Divider” color=”#ffffff” show_divider=”off” height=”10″ /][et_pb_slider admin_label=”Slider” show_arrows=”on” show_pagination=”on” auto=”on” parallax=”off” auto_speed=”3000″][et_pb_slide background_image=”https://www.cherrybiotech.com/wp-content/uploads/2014/04/CherryTemp-microfluidic-platform-for-microscopy-temperature-control-3.jpg” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][et_pb_slide background_image=”https://www.cherrybiotech.com/wp-content/uploads/2014/04/microfluidic-chip-for-live-cell-imaging.jpg” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][et_pb_slide background_image=”https://www.cherrybiotech.com/wp-content/uploads/2014/04/confocal-inverted-microscope.png” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][et_pb_slide background_image=”https://www.cherrybiotech.com/wp-content/uploads/2014/04/CherryTemp-and-CherryLoop-temperature-control-for-microscopy-2.jpg” background_color=”#ffffff” alignment=”center” background_layout=”dark” /][/et_pb_slider][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off”][et_pb_row][et_pb_column type=”1_4″][et_pb_blurb admin_label=”Blurb E njoy unprecendeted performance” title=”Enjoy the performance” url=”https://www.cherrybiotech.com/?p=312″ url_new_window=”off” image=”https://www.cherrybiotech.com/wp-content/uploads/2014/03/icon_rook2.png” alt=”Enjoy unprecendeted performance” animation=”off” background_layout=”light” text_orientation=”center”]

CherryTemp is the fastest temperature control in the 5-45°C range
[button color=”silver” link=”https://www.cherrybiotech.com/?p=312″] Learn more[/button]

[/et_pb_blurb][/et_pb_column][et_pb_column type=”1_4″][et_pb_blurb admin_label=”Blurb Work with any type of live cell” title=”Work with any of live cell” url_new_window=”off” image=”https://www.cherrybiotech.com/wp-content/uploads/2014/03/icon_grid-3×32.png” alt=”Work with any type of live cell” animation=”off” background_layout=”light” text_orientation=”center” url=”https://www.cherrybiotech.com/?p=1346″]

CherryTemp is adapted to all types of live cells including S. pombe and C. elegans
[button color=”silver” link=”https://www.cherrybiotech.com/?p=1346″] Learn more[/button]

[/et_pb_blurb][/et_pb_column][et_pb_column type=”1_4″][et_pb_blurb admin_label=”Blurb Start experiments in minutes” title=”Start experiments in minutes” url_new_window=”off” image=”https://www.cherrybiotech.com/wp-content/uploads/2014/03/icon_hourglass2.png” animation=”off” background_layout=”light” text_orientation=”center” url=”https://www.cherrybiotech.com/?p=896″]

CherryTemp is easy to use so that you focus on your research, not the instrument
[button color=”silver” link=”https://www.cherrybiotech.com/?p=896″] Learn more[/button]

[/et_pb_blurb][/et_pb_column][et_pb_column type=”1_4″][et_pb_divider admin_label=”Divider” color=”#ffffff” show_divider=”off” height=”15″ /][et_pb_text admin_label=”Text C elegans” background_layout=”light” text_orientation=”center” module_class=”elegans”]

C. elegans

See another example of temperature control with thermosensitive mutants

[button color=”silver” link=”https://www.cherrybiotech.com/?p=1113″] Application note[/button]

[/et_pb_text][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section fullwidth=”off”][et_pb_row][et_pb_column type=”4_4″][et_pb_cta admin_label=”Call To Action” title=”Want to know more about the system?” button_url=”https://www.cherrybiotech.com/contact-us” button_text=”Contact us” background_color=”#a40000″ use_background_color=”on” background_layout=”dark” text_orientation=”center”]

contact@cherrybiotech.com

[/et_pb_cta][/et_pb_column][/et_pb_row][/et_pb_section]

This is a unique website which will require a more modern browser to work! Please upgrade today!

Social Media Auto Publish Powered By : XYZScripts.com