Centrosome regulation in C. elegans embryo first division

This application note shows Centrosome regulation in C. elegans embryo first division using CherryTemp for Temperature control and temperature shifts .

Experimental conditions

C. elegans zyg-12 t.s mutant temperature shift

Control and zyg-12 embryos shifted to 24°C at anaphase onset (red: DNA,  green: alpha-tubulin).
In the zyg-12 mutant centrosomes fail to associate to the nucleus.

Courtesy of Anne Pacquelet (“Membrane traffic and Polarity in C. elegans” Grégoire Michaux’s lab, IGDR)

Divisions were recorded using a microscope MRic (Microscopy Rennes Imaging Center). Temperature control and tempertaure shifts were performed using Cherry Temp.