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Therma Flow: protocol for mammalian cells
The following protocol explains how to use the temperature controller CherryTemp with the Therma Flow chip for medium perfusion when adherent mammalian cells are ...
Read moreTherma Flow: Lectin protocol for yeast
The following protocol explains how to use the temperature controller CherryTemp with the Therma Flow chip for medium perfusion when yeast cells are mounted ...
Read moreProtocol for in vitro imaging of molecular assemblies
Mounting method for in vitro imaging of molecular assembly using CherryTemp This protocol shows how to create a chamber on a glass coverslip to image ...
Read moreProtocol for Drosophila larval brains
Mounting method for third larval brains of D. Melanogaster This protocol shows how to mount Drosophila 3d instar larval brains using a CherryTemp chip. CherryTemp allows ...
Read moreLectin protocol for yeast
Concanavalin A mounting protocol The following protocol explains how to use the temperature controller CherryTemp when yeast cells are mounted on lectin coated slides. Yeast ...
Read moreProtocol for Drosophila optic lobes
Mounting method for D. Melanogaster optic lobes This protocol is for short term imaging (up to 2 hours) of optic lobes. Spacers can replace standard methods which ...
Read moreProtocol for Drosophila pupa
Mounting method for Drosophila pupa With this protocol, spacers of multiple thicknesses can be used to adjust to the diameter of the pupa. Spacers thicknesses ...
Read moreProtocol for Drosophila larva
Mounting method for Drosophila larva With this protocol, spacers of multiple thicknesses can be used to adjust to 1st, 2nd and 3d instar larvae. Spacers ...
Read moreProtocol for Drosophila with gas permeable chamber
Mounting method for Drosophila larval brain With this protocol, a gas permeable chamber is created by means of a double-sided tape. The permeable chamber allows ...
Read moreAgarose pad protocol for yeast
Agarose pad mounting protocol for yeast For full efficiency of the CherryTemp temperature controller, samples must be placed close to the CherryTemp thermalisation chip (<500µm). ...
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