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This application note shows Centrosome regulation in C. elegans embryo first division using CherryTemp for Temperature control and temperature shifts .
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Experimental conditions
C. elegans zyg-12 t.s mutant temperature shift
Control and zyg-12 embryos shifted to 24°C at anaphase onset (red: DNA, green: alpha-tubulin).
In the zyg-12 mutant centrosomes fail to associate to the nucleus.
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Courtesy of Anne Pacquelet (“Membrane traffic and Polarity in C. elegans” Grégoire Michaux’s lab, IGDR)
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[embedyt] https://www.youtube.com/watch?v=cw-L7yAUb7o[/embedyt]
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Divisions were recorded using a microscope MRic (Microscopy Rennes Imaging Center). Temperature control and tempertaure shifts were performed using Cherry Temp.
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