Our Blog

Centrosome regulation in C. elegans embryo first division

This application note shows Centrosome regulation in C. elegans embryo first division using CherryTemp for Temperature control and temperature shifts .

Experimental conditions

C. elegans zyg-12 t.s mutant temperature shift

Control and zyg-12 embryos shifted to 24°C at anaphase onset (red: DNA,  green: alpha-tubulin).
In the zyg-12 mutant centrosomes fail to associate to the nucleus.

C. elegans control and zyg-12 embryos shifted to 24°C at anaphase onset

Control

C. elegans control and zyg-12 embryos shifted to 24°C at anaphase onset

zyg-12

IGDR

Courtesy of Anne Pacquelet (“Membrane traffic and Polarity in C. elegans” Grégoire Michaux’s lab, IGDR)

Discover CherryTemp

References

Divisions were recorded using a microscope MRic (Microscopy Rennes Imaging Center). Temperature control and tempertaure shifts were performed using Cherry Temp.

Tags:

This is a unique website which will require a more modern browser to work! Please upgrade today!