Microtubules depolymerisation in Drosophila larval brain related contents
When we developed our CherryTemp fast heater/cooler back in 2011, the very first application was microtubule depolymerisation in fission yeast. The sensitivity of microtubules to temperature and the ability to dynamically control their elongation thanks to temperature changes is clearly organism dependent. For the first time under a microscope, CherryTemp has been used to trigger microtubule depolymerisation in Drosophila larva brains. Enjoy the time-lapses!
Microtubule depolymerization by cold in Drosophila third instar larval brains
Drosophila third instar larval brains expressing a microtubule marker (Jupiter-mCherry) were mounted on a CherryTemp slide. The sample was initially held at 20°C and then rapidly switched to 5°C. Microtubule depolymerisation was observed for 370 seconds before the sample was rapidly taken back to 20°C to observe microtubule repolymerisation…. READ FULL CONTENT
Imaging Drosophila third instar larval brain cells expressing Jupiter-mCherry as a microtubule marker
Drosophila brains expressing a centriole marker (GFP-PACT) and a microtubule marker (Jupiter-mCherry) were dissected from third instar larvae in PBS and placed in a 10ml drop of Live Cell Imaging Solution (Life Technologies, A14291DJ) on top of the fluidic chamber….READ FULL CONTENT