Is CherryTemp compatible with both air and immersion objective lenses? How do you compensate for the heat loss through both the specimen and also oil from the objective?

Is CherryTemp compatible with both air and immersion objective lenses? How do you compensate for the heat loss through both the specimen and also oil from the objective?

Our innovation relies on coupling a temperature calibration of our systems and a smart algorithm taking into account all the sources of heat lost: the room temperature fluctuations and the heat sink played by the objective lens.

We performed several experiments and set up two specific calibrations whether an immersion objective was in contact with the coverslip or not. Our software integrates these data and calculates a correcting factor depending on the type of objective lense you will use for your experimentation. As a consequence, the temperature you set in the software is exactly the one that is experienced by your cells.

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Heat sink played by immersion oil objectives

 

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