Is there any risk for losing the focus point when shifting from one temperature to another?

Is there any risk for losing the focus point when shifting from one temperature to another?

You are right that a change in temperature will create a focus drift. This is a physical law that we cannot change. However, your microscope might be equipped with an autofocus system. We performed experimentations with microscopists to troubleshoot their hardware autofocuses and they were able to readjust the focus after drastic temperature changes.

Alternatively, as this delta-focus variation is constant between your two temperatures the focus change is predictable and you could save the proper focus point before starting the experiment.

Tags:

This is a unique website which will require a more modern browser to work! Please upgrade today!