Fibroblasts were grown and cultivated for 7 days
AIMS
→ Improve IPSc derived cardiomyocytes differentiation
→ Establish a Duchenne’s disease model on a chip
→ Combine electrical stimulations with biochemicals and bio-inductive extra cellular matrix
FEATURES
→ Temperature : 37°C
→ Gas : normoxia, 5% CO2
→ Perfusion mode : pulse
→ Culture unit : custom microfluidic chip
Cardiomyocytes derived from iPSCs were cultured for 4 days
Cardiomyocytes derived from iPSCs were cultured for 10 days. Contracting cardiomyocytes were obtained after 7 days of culture
RESULTS
→ Electrodes combination, biochemical and cell culture chamber in a tailored chip
→ Electrophysiological current detection to follow the differentiation stage
→ Myofilament immunofluorescence: sarcomere detection
PERSPECTIVE
→ Standardize & Industrialize the protocol
→ Duchenne’s disease mechanism deciphering and drug testing For more information head over to our new website innovation.cherrybiotech.com
https://innovation.cherrybiotech.com/organs-on-a-chip/heart-on-a-chip-state-of-the-art
This project has received funding from H2020-MSCA-RISE2017 program under grant agreement No. 778354
