Heater/Cooler for Drosophila
Ultrafast temperature changes
Stable & precise : long term imaging
Fits upright & inverted hr microscopes
Dedicated mounting systems for different developmental stages :
Embryo, 1st,2nd & 3rd instar larva, pupa, adult tissue explants.
Application using CherryTemp
Microtubule depolymerization by cold in Drosophila third instar larval brains
Drosophila third instar larval brains expressing a microtubule marker (Jupiter-mCherry) were mounted on a CherryTemp slide. The sample was initially held at 20°C and then rapidly switched to 5°C. Microtubule depolymerisation was observed for 370 seconds before the sample was rapidly taken back to …Read More…
Courtesy of Paul T. Conduit lab, University of Cambridge, UK. http://conduitlab.zoo.cam.ac.uk/
Imaging Drosophila third instar larval brain cells expressing EB1-GFP as a growing microtubule marker
Drosophila brains expressing the microtubule plus-end marker EB1-GFP were dissected from third instar larvae and mounted on a CherryTemp chip. The chip was then attached to the CherryTemp system and mounted on a Leica DM IL LED inverted microscope controlled by μManager software and coupled to a RetigaR1 monochrome camera …Read More…
Courtesy of Paul T. Conduit lab, University of Cambridge, UK. http://conduitlab.zoo.cam.ac.uk/
Drosophila mounting Methods
Protocol for a sample mounting system using mutiple spacers
This video shows how to use multiple spacers according to the thickness of the tissue ot whole organism being imaged. To mount with a CherryTemp chip for thermalization. The spacers prevent evaporation.
Protocol for a sample mounting system using double-side tape
This video shows how to use a double-side tape to create a chamber for your sample, to be pasted to a CherryTemp chip for thermalization. This material prevents evaporation and it is suitable for long term imaging.
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Testimonials & Universities using CherryTemp
Dr. Emmanuel Derivery
MRC Lab of Molecular Biology, UK
“CherryTemp is a one-of-a kind device for the study of thermosensitive mutants and cytoskeleton dynamics. Its flexibility makes it very easy to adapt to various geometries, from cells to tissue explants or even whole animals !”
Visit the Derivery Lab
Dr. Stéphanie Dutertre
BIOSIT LABORATORY, France
“The Cherry Temp is perfect for use in imaging core facilities. Its design allows its installation on a wide panel of upright and inverted microscopes and easy training of users thus, promoting development of innovating research project. The customer service is always helpful and responsive.”
Visit Dr Dutertre’s core facility MRic
System photo gallery
CherryTemp fits a wide range of high resolution microscopes.
