Yeast cold-shock microtubules depolymerization with CherryTemp

We depolymerized S. pombe microtubules while imaging the cells (gamma-tubulin GFP) with a cold shock at 5°C (25°C to 5°C in less than 10 sec). We then repolymerized by shifting back the temperature within seconds to 25°C.

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Temperature shift to image S.pombe temperature sensitive mutants

Application example: Block and release of cdc25-22 S. pombe ts mutants.

CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp

Figure 1, cdc25 ts S. pombe reversible control of mitosis arrest by CherryTemp


Figure 2, CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp

Figure 2, CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp, We thank Damien Coudreuse’s lab (Julien Babic, PhD st. – IGDR, Rennes) for credits.

cdc25-22 mutants were blocked at the G2/M transition for 4 hours at restrictive temperature (36.5 °C). Synchronous release into the cell cycle was achieved by a quick shift (10 seconds) into permissive temperature (25°C). Septation index shows the high synchronicity in the release.


CherryTemp™ – Heater/Cooler system for Yeast

DYNAMIC & FAST : 10 SECONDS TEMPERATURE SHIFTS

The fastest heater-cooler for microscopy.

STABLE & PRECISE IN THE RANGE OF 5°C TO 45°C

First software algorythm compensating room temperature variation.

FOLLOW TS MUTANTS WITH ACCURACY

Budding and fission yeast live cell imaging.

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Relevant Publications for Yeast Application

Medium renewal and temperature control for live-cell imaging

Medium renewal and temperature control for live-cell imaging

(Open Biology, 2016) Summary | Monitoring cellular responses to changes in growth conditions and perturbation of targeted pathways is integral to...
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Temperature control of thermosensitive protein regulation

Temperature control of thermosensitive protein regulation

(Developmental cell, 2009) Summary | The spindle midzone—composed of antiparallel microtubules, microtubule-associated proteins (MAPs), and motors—is the structure responsible for microtubule...
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Fast microfluidic temperature control during high resolution live cell imaging

Fast microfluidic temperature control during high resolution live cell imaging

(Lab on Chip, 2011) Summary | One major advantage of using genetically tractable model organisms such as the fission yeastSchizosaccharomyces pombe is...
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Imaging of mitochondria positioning using fast temperature regulation

Imaging of mitochondria positioning using fast temperature regulation

(Current Biology, 2011) Summary | Mitochondria form a dynamic tubular network within the cell. Proper mitochondria movement and distribution are critical...
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