CherryTemp™ – Heater/Cooler stage for Yeast

DYNAMIC & FAST : 10 SECONDS TEMPERATURE SHIFTS

The fastest heater-cooler for microscopy.

STABLE & PRECISE IN THE RANGE OF 5°C TO 45°C

First software algorythm compensating room temperature variation.

FOLLOW TS MUTANTS WITH ACCURACY

Budding and fission yeast life cell imaging.

You can now control in real-time your favorite molecular processes, observe dynamic events linked to temperature on live and discover unthought mechanisms.

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Heat and cool down your samples in the 5 to 45°C range

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Temperature shift to image S.pombe mutants

Application example: Block and release of CDC25 S. pombe ts mutants.

CDC25-22 h+ S. pombe mutants where first blocked for 4 hours at the restrictive 36.5°C temperature (Pict. A). Division was bloqued until temperature had been quickly changed (in 10 seconds) to the permissive 25°C. No septum could be observed  after 15 minutes of release (Pict. B, 0%) compared to a large 93% proportion of cells showing a septum after 90 minutes (Pict. C).

Observation was performed on a Zeiss Axio Observer Z1, fully motorized inverted microscope with an ASI PZ-2000FT XY-Z Piezo stage and Zeiss Definite Focus using a 63X oil objective (Zeiss Plan-Apochromat).

We thank Damien Coudreuse’s lab (Julien Babic, PhD st. – IGDR, Rennes) for credits.

CDC25-ts-S-pombe-block-and-release-by-CherryTemp

Temperature modification from 25°C to 37°C resulting in a reversible arrest of mitosis.

Application example: Mitosis arrest of yeast CDC25 ts mutants.

CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp

S. pombe thermosensitive mutants (CDC25 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of yeast samples between 25°C and 37°C resulting in a reversible arrest of mitosis.

Yeast cold-shock microtubules depolymerization with CherryTemp

We depolymerized S. pombe microtubules while imaging the cells (gamma-tubulin GFP) with a cold shock at 5°C (25°C to 5°C in less than 10 sec). We then repolymerized by shifting back the temperature within seconds to 25°C.

How long is it to install cherry temp? From yeasts to observation in 30 seconds

Change the temperature of your sample within seconds during live-cell imaging

Initially developed for PCR military application, the world fastest temperature controller for microscopy is now available for live-cell imaging.

Get benefit of our robust and precise temperature calibration. Our software continuously integrates changes of your room temperature. It also takes into account the heat sink played by immersion objective lenses.

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Methods

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Scientific notes

Testimonial

“The Cherry Temp is perfect for use in imaging core facilities.  Its design allows its installation on a wide panel of upright and inverted microscopes and easy training of users thus, promoting development of innovating research project. The customer service is always helpful and responsive”  visit Stephanie Dutertre core facility MRic

Stéphanie Dutertre - Instrument core facility manager

Biosit laboratory, CNRS 3480 - INSERM 018, MRic

“The Cherry system is now in full use in my lab and it is working beautifully on its own. The Cherry system is great for stably maintaining temperature on our microscopes”Visit the Canman Lab 

Dr. Julie Canman

CANMAN LAB, Columbia University Medical Center

Our videos

Publications

C. elegans chromosome segregation: microtubule pushing

C. elegans chromosome segregation: microtubule pushing

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Inhibition of microtubule assembly using fast temperature changes

Inhibition of microtubule assembly using fast temperature changes

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Medium renewal and temperature control for live-cell imaging

Medium renewal and temperature control for live-cell imaging

(Open Biology, 2016) Summary | Monitoring cellular responses to changes in growth conditions and perturbation of targeted pathways is integral to...
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Real-time qPCR using CherryTemp on-a-chip

Real-time qPCR using CherryTemp on-a-chip

(Lab on Chip, 2016) Summary | To control future infectious disease outbreaks, like the 2014 Ebola epidemic, it is necessary to...
Read More

GET A QUOTE OR TECHNICAL INFORMATION

Heat and cool down your samples in the 5 to 45°C range

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