CherryTemp case study

Membrane traffic and Polarity in C. elegans

Courtesy of Anne Pacquelet (Grégoire Michaux’s lab, IGDR)

check C. elegans pack

Experimental conditions

C. elegans zyg-12 t.s mutant temperature shift

Control and zyg-12 embryos shifted to 24°C at anaphase onset (red: DNA,  green: alpha-tubulin).

In the zyg-12 mutant centrosomes fail to associate to the nucleus.

Divisions were recorded using a microscope MRic (Microscopy Rennes Imaging Center). Temperature control and tempertaure shifts were performed using Cherry Temp.

Control

zyg-12

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Cherry Temp

CherryTemp,  

Robuste and user-friendly

Temperature range for your sample: from 5°C to 45°C

Precision:  at 0.1°C

Delay for temperature shifts: 10 seconds

Types of microscope:  any microscopes

CherryCare service available for Microscope facility

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