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Lectin (Concanavalin A) mounting protocol for yeast live-cell imaging

The following protocol explains how to use the temperarture controller CherryTemp when yeast cells are mounted on lectin coated slides.

Yeast cells are deposited on lectin coated coverslips and our dedicated spacers allow to simply place the CherryTemp chip on top.

Lectin helps to reduce cell movement for optimal imaging and CherryTemp allows for an accurate temperature control without disturbing cells.

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Figure 1: Pipet a drop of lectin (Concanavalin A, Con A) on a high resolution glass coverslip

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Figure 2: Allow the liquid to evaporate under the hood

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Figure 3: Top the coverslip with our dedicated spacer

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Figure 4: Drop the yeast sample (up to 15µl) centered in the spacer hole. Prior concentration of cells is recommended to achieve an optimal cell density.
Important: medium must not touch the sides of the spacer

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Figure 5: Top with the CherryTemp chip (engravements facing up, coverslip and liquid microfluidic chamber facing the sample)

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Figure 6: Place the mounted slide on either an upright or an inverted configuration by simply rotating 180°

Methods adapted from P. Tran lab (Institut Curie) and from our user’s feedback.

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