The vascular niche in next generation microphysiological systems

Introduction

As a novel technology for in vitro toxicity testing platforms in recent years, Microphysiological Systems (MPS) have been actively developed. MPS are cultural techniques for the rebuilding of human organs and tissue functions in a confined environment for the development of miniature human experimental systems. As methods for in vitro toxicity evaluation of next-generation, MPS offer considerable promise.

The phrase ‘vascular niche’ refers to a region rich in blood arteries where endothelial cells and mural cells, such as pericytes and smooth muscle cells, create a microenvironment that influences the activity of various stem and progenitor cells.

Learn more in the review below.

Abstract

The authors state that “In recent years, microphysiological system (MPS, also known as, organ-on-a-chip or tissue chip) platforms have emerged with great promise to improve the predictive capacity of preclinical modeling thereby reducing the high attrition rates when drugs move into trials.

While their designs can vary quite significantly, in general MPS are bioengineered in vitro microenvironments that recapitulate key functional units of human organs, and that have broad applications in human physiology, pathophysiology, and clinical pharmacology. A critical next step in the evolution of MPS devices is the widespread incorporation of functional vasculature within tissues.

The vasculature itself is a major organ that carries nutrients, immune cells, signaling molecules and therapeutics to all other organs. It also plays critical roles in inducing and maintaining tissue identity through expression of angiocrine factors, and in providing tissue-specific milieus (i.e., the vascular niche) that can support the survival and function of stem cells. Thus, organs are patterned, maintained and supported by the vasculature, which in turn receives signals that drive tissue specific gene expression.

In this review, we will discuss published vascularized MPS platforms and present considerations for next-generation devices looking to incorporate this critical constituent. Finally, we will highlight the organ-patterning processes governed by the vasculature, and how the incorporation of a vascular niche within MPS platforms will establish a unique opportunity to study stem cell development.”

Source

Ewald, M. L., Chen, Y. H., Lee, A. P., & Hughes, C. C. W. (2021). The vascular niche in next generation microphysiological systems. Lab on a Chip, 21(17), 3244–3262. https://doi.org/10.1039/d1lc00530h

Leave a Comment

Your email address will not be published. Required fields are marked *