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Fission yeast cells: temperature shifts from 5°C to 30°C

In 2016, we co-published the following video with Zeiss. It shows how the Definite Focus 2 module is able to compensate an impressive 50µm loss-of focus induced by temperature dilatation.

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Fission yeast cells were subjected to temperature shifts from 5°C to 30°C.

Loss of focus occurs as a consequence of dilatation due to temperature changes.

This video shows how the Zeiss Definite Focus 2 module is able to compensate a 50µm loss-of focus. Samples were imaged on a Zeiss LSM 800 microscope.

Video co-published between Cherry Biotech and Zeiss France during the Yeast Symposium in Toulouse 2016.

Full application note :

Microfluidic devices and long-term live-cell imaging: CherryTemp & the Zeiss Definite Focus 2

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