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Mitosis and ts mutants

Temperature-sensitive mutant yeast

During S. pombe division, the CDC25 protein is required for mitosis completion. At 25°C the CDC25 protein is functional (Fig. 1a) but over 37°C it becomes non-functional or inactivated (Fig. 1b). Such a mutant is called “thermosensitive” and as mitosis only occurs at 25°C, this temperature is termed “permissive”. In these experiments, we used the CherryTemp precise temperature control, yet hitherto impossible to achieve, to impact S. pombe physiology during cell division.

Fig.1: CDC25 protein is fonctional at 25°C (a) but becomes inactivated at 37°C (b)

Fig.1: CDC25 protein is fonctional at 25°C (a) but becomes inactivated at 37°C (b)

CherryTemp induced cell cycle phenotype

During our experimentation, S. pombe thermosensitive mutants (CDC25 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of yeast samples between 25°C and 37°C resulting in a reversible arrest of mitosis.

Fig 2: CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp Yeast division occurs normally at 25°C (a) but is suddenly stopped when temperature is quickly shifted to 37°C (b). Mitosis only restarts when temperature is set back to 25°C (c, d, e, f)

Fig 2: CDC25 ts S. pombe reversible control of mitosis arrest by CherryTemp
Yeast division occurs normally at 25°C (a) but is suddenly stopped when temperature is quickly shifted to 37°C (b). Mitosis only restarts when temperature is set back to 25°C (c, d, e, f)


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