FULLY DIFFERENTIATED SKIN ON CHIP

Reijnders CMA,…Gibbs S,  Development of a Full-Thickness Human Skin Equivalent In Vitro Model Derived from TERT-Immortalized Keratinocytes and Fibroblasts,  Tissue Eng Part A. 2015 Sep 1; 21(17-18): 2448–2459.   

AIMS

→ Obtain a fully differentiated healthy skin equivalent

→ Build a fully differentiated Melanoma on a chip

→ Detect CTC in the flow stream

→ Maintain the system for several weeks

FEATURES

→ Temperature : 37°C

→ Gas : normoxia, air-liquid interface

→ Perfusion mode : pulse & continuous

→ Culture unit : 6 well multi well & trans well

RESULTS

→ Implementation of the protocol to fully differentiated skin equivalent

→ Establishment of the melanoma on a chip: ongoing

→ Implementation of optical detection of tagged CTC in the flow stream (signal detection based on GFP)

PERSPECTIVE

→ Testing anti-migration drugs