LIVER BIOPSY ON CHIP

AIMS

→ Increase biopsies viability in long term culture

→ Imaging and biochemical quantification of drug effect

→ Interface blood vessel/hepatocyte

→ DILI assessment based on biochemical marker and morphometric read-out

FEATURES

→ Temperature : 37°C

→ Gas : normoxia & hypoxia

→ Perfusion mode : pulse

→ Culture unit : 24 well multi well & 2 custom microfluidic chip

RESULTS

→ Metabolic functions maintain over 7 days (CYP450, EROD…)

→ First morphometric criteria acquired : necrosis assessment (edges, center)

→ Biochemical markers and DILI assessment ongoing

PERSPECTIVE

→ Going to super-resolution to image eventual effect of drugs on endotelial nanopores structures (supposed target of toxicity)

→ Going above 7 days to mimic chronic exposure (several plasmatic peaks patterns..)

To learn more head over to our new innovation website for Cubix –  Liver Biopsy on a chip