LIVER BIOPSY ON CHIP
AIMS
→ Increase biopsies viability in long term culture
→ Imaging and biochemical quantification of drug effect
→ Interface blood vessel/hepatocyte
→ DILI assessment based on biochemical marker and morphometric read-out
FEATURES
→ Temperature : 37°C
→ Gas : normoxia & hypoxia
→ Perfusion mode : pulse
→ Culture unit : 24 well multi well & 2 custom microfluidic chip
RESULTS
→ Metabolic functions maintain over 7 days (CYP450, EROD…)
→ First morphometric criteria acquired : necrosis assessment (edges, center)
→ Biochemical markers and DILI assessment ongoing
PERSPECTIVE
→ Going to super-resolution to image eventual effect of drugs on endotelial nanopores structures (supposed target of toxicity)
→ Going above 7 days to mimic chronic exposure (several plasmatic peaks patterns..)
This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 766181, project “DeLIVER”.