Medium injection & medium perfusion solutions for microfluidics
This review is related to medium injection and medium perfusion solutions for microfluidics. It explains differents methods and techniques (active and passive) as an introduction to perfusion for live cell imaging.
Passive medium injection techniques in microfluidics
Capillary forces
Capillary effects are key forces at small scales, in the range of millimeters to micrometers. They are present when one liquid is in contact with any other gas, liquid or solid. That contact or interface rich to a surface tension which is the force created between both surfaces of the different components when they get in touch. The creation of a new interface has a cost of energy that can be expressed in the following equation: E=σA. Where σ is the surface tension measured in N/m while the A is the area of the interface or contact between the two components and E is the force created. As an example, surface tension is the reason why liquid droplets are spherical because they tend to reduce their surface to reduce the forces needed to be stable (1).
Fig1: Scheme of capillarity forces at solid-liquid, solid-gas or liquid-gas interfaces
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Long-term medium perfusion, change and drug injection
Reference
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Written by Pablo Salaverria
PHD STUDENT | INNOVATION UNIT | H2020-MSCA-ITN-DIVIDE
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