Organotypic brain slice culture : Organotypic brain slice culture : Prospect of a human model in preclinical drug development
Brain: No-human in-vivo model
The human brain is one of the most convoluted organs. Contemporaneously,in-vivoand in-vitronon-human models are unraveling the mechanism of the complex brain signaling and additionally are being used to identify the therapeutic agents.
Several vertebrates including zebrafish, mice, and rat are applied to prototype human neurodegeneration. Neuro-disorders are either replicated chemically or genetically imitated through mutation in selected no-human models. However, many drug contenders failed to meet the translation from no-human to human model after the effective screening in the animal. Often, no-human models partially exhibit the etiology and symptomatic traits of stimulated or mutated diseases. As the anatomy, physiology, and biochemistry of the human brain are substantially different from the modeled vertebrates, henceforth the comparison concerning no-human and human model is impractical.
In-vitro analysis of human brain cells: clinical applications
Generally, in-vitro humanmodels encompass the individual or mixed culture of primary brain cells separated from different age human or preserved cell lines. Human brain cell culturing involves rigorous and laborious biological protocols. The protocols have been devised in two phases: 1) protocol cell isolation includes the cell dissociation measures from sliced tissue, 2) protocol cell culture relates to the developing etiquettes for the considered neurological disorder. The following animations summarized the studies using primary cultures and cell lines of neurons, microglia, astrocytes, and oligodendrocytes. Referenced studies correlated with brain cell culture are being utilized to unveil the processes including inflammation, neurotoxicity, and neuroprotection, additionally, to screen for pre-clinical drugs to treat neurodegenerative disorders.
Limitations of dissociate human culture compensated with slice culture
The chief disadvantage of the dissociate culture is associated with brain anatomical and cellular interactions, which cannot be replicated easily. Moreover, the number of autopsies is declined day by day; hence, the availability of the brain tissue is limited. In this scenario, more often, researchers find that the yield of brain cells is relatively low after tissue dissociation.
In contrast, the advancement in slice culture method enables limited anatomical and cellular interactions and offers a model nearer to the in vivo situation. Additionally, Verwer 2002 claims that brain tissue obtained from an autopsy can be retained for a more extended period (up to 78 days), and further can be manipulated.
Apprehension from upcoming technologies
Further modification in slice culture method will deliver a robust system for analyzing the fundamental biology of human brain cells and their wirings. The system will enable the understanding of basic procedures of brain cell death, cell repairing, and testing novel protocols for brain disorders. Slice culture can be utilized to explore electrical properties and synaptic functioning of the human brain via applying electrophysiological approaches. These cultures could be established as standard studies to unfold mechanisms of neuroinflammation, the death of human neurons, and glial scar formation.
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