SPHEROID HUMAN KIDNEY CANCER ON CHIP

Cherry_Biotech_kidney_spheroid_cancer_on_chip

AIMS

→ Characterization and quantification of antimigration and antiproliferative effects of several molecules, mimicking acute exposure.

→ Detection of specific biomarkers

FEATURES

→ Temperature : 37°C

→ Gas : normoxia 5% CO2

→ Perfusion mode : pulse & continuous

→ Culture unit : custom microfluidic chip

Cherry_Biotech_kidney_spheroid_cancer_on_chip_2

Cherry_Biotech_kidney_spheroid_cancer_on_chip_3

RESULTS

Spheroids viable during a 5 days experiment with perfusion of media

→ EC-50 of each molecule has been determined

→ Live detection and following of the biomarkers inside the chip

Cherry_Biotech_CubiX

Cherry_Biotech_Spheroid_culture

PERSPECTIVE

→ Mimicking chronic exposure

→ Multiplexing of chips to identify drug effect on other organs

Conseil-regional-de-Bretagne

 

Project financed by the Brittany region through the call for technology transfer projects