Temperature control for microscopy imaging: heater/cooler microscope stages

Introduction

Biological processes are very sensitive, and respond rapidly to temperature variation. Performing live-cell imaging implies to maintain the cells temperature as long as image acquisition is required. Several temperature controllers are available on the market, all have advantages and caveats and depending on your experimental needs they may meet your needs. Microscope stage heater/cooler is one way to control or shift the temperature of a sample.

Precision-30x28 How does it work?

Microscope stages heater/cooler are a substitute to your regular stage. They have a very high thermal capacity and use resistive heaters or Peltier devices to heat or cool down the sample. Temperature controlled microscope stages change the temperature by thermal diffusion through the glass slide or metal heat conducting bridge, which reduce the formation of thermal gradient on the sample. Microscope stages temperature range from -20C to 100C and temperature-shift occurs within minutes.

Precision-30x28 Advantages

 Large temperature range

-20C to 100C, the use of a Peltier element allows microscope stage to reach température below ambient. This is an important features, notably for scientists studying microtubules assembly or vesicular transport.

 Fast temperature switch

Since the surface to thermalize is reduced to the microscope stages, the speed of température Switch is much faster than for an incubation box. Depending on the power of the temperature actuators, the temperature switch can be achieved in 2 to 3 min, but overshoots of several °C should be expected when system speed is increased.

 Easy access to the cells

Depending on the choosen setting, manipulator can have access to his cells during his experiment. Possibility to add drugs or medium without changing the objective focus or stopping the recording of the ongoing experiment.

Precision-30x28Disadvantages

Poor temperature uniformity across the sample

Microscope stages control sample temperature using thermal diffusion. The heat transfer from the temperature control device to the sample can generate thermal gradient up to several degree on the sample.

 Requires objective heater

The use of oil or water immersion objectives will require the use of an objective heater. Indeed, when the objective, the oil, and coverslip make contact, the objective acts as a heat sink leading to 5-7C in sample temperature (room with a view). Also, a strong gradient can arise if the microscope stage temperature shift response is faster or slower than with the collar system.

 Requires dehumidifier

While microscope stages allow to reach temperature below ambient, it is recommend to use a dehumidifier to prevent condensation.

 Lack of active cooling system

Since there is no active cooling system, samples are subjected to strong temperature increase when using DIC illumination or during extended observation under epi-fluorescence microscopy. There is also a heat diffusion to nearby microscope element which may affect the objective focus.

 Mono temperature control

Only one temperature can be set on the microscope stage temperature controller. While other systems allows to set two température at once and shift from one to the other ultra-rapidly.

 

CherryTemp is the fastest temperature control in the 5-45°C range
CherryTemp is adapted to all types of live cells including S. pombe and C. elegans
CherryTemp is easy to use so that you focus on your research, not the instrument

CherryTemp heater/cooler for live-cell imaging

Dynamic & fast : 10 seconds temperature shifts


Stable & Precise : Long term stability


Discover the C. elegans heater/cooler

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