The development of new techniques enabled scientists to screen more easily for C. elegans temperature sensitive mutants and by shifting temperature from permissive to sensitive background, thus precisely question the role of protein, and its absence thereof, during development, cell division, neuron formation .
Pioneer work in C. elegans genetics and developmental biology has been initiated by Sydney Brenner since 1974. Taking advantage of the worm small number of cells, Sulston and Horvitz, back in 1977, initiated thorough cell lineage analyses. Forty years later, scientists can precisely document every single cell fate and genealogy cell tree (see figure 1) have been established.
The sum of the next-generation 3D cells culture technologies.
C. elegans temperature sensitive mutants
Temperature sensitive mutants represent a powerful tool to study many aspect of worm biology. Due to the technical improvements, the creation of thermo-sensitive worms has become a very easy task. For example, by combining a heat-shock protein promoter to any genes of interest allows researcher to precisely control gene activation.
The first genetic studies done in relation with the thermo sensitivity used mutants that had defects in the chemotaxis. The study focused on finding genes that are sensitive to temperatures changes .
As an example, a group of scientist from the team of M. Chatzigeorgiou  found that the exposure of C. elegans to low temperatures, from 20°C to 15°C, provokes changes in the genetic expression of trpa-1. The genetic variations lead to altered translation of the TRP-1 protein and a consequent cold-sensitive, not selective ion current.
There are more methods to achieve temperature dependence in this transparent nematode. In C. elegans, the AFD neurons  need a cGMP-dependent network of molecules to control and regulate the ion channel. Inside this network, the temperature is the one that regulates the concentration of cGMP in the cytoplasm. The concentration level of this molecule is the one that sets how many cGMP gated ion channels are open at a certain temperature .
ASI chemosensory neurons have relation with thermotaxis under very specific conditions . The response of ASI to temperature variations is dependent on AFD as it can be seen in the Figure 2.There are more methods to achieve temperature dependence in this transparent nematode. In C. elegans, the AFD neurons  need a cGMP-dependent network of molecules to control and regulate the ion channel. Inside this network, the temperature is the one that regulates the concentration of cGMP in the cytoplasm. The concentration level of this molecule is the one that sets how many cGMP gated ion channels are open at a certain temperature .
As examples, the Alzheimer, Parkinson, ALS and Huntington’s diseases are being studied in C. elegans . Both in ALS and Huntington’s diseases, it has been shown that temperature sensitive mutant genes can act as modifiers to proteins misfolding and aggregation toxicity.
Optimizing the use of C. elegans temperature-sensitive mutants
The possibility of growing the worms at different temperatures gives the chance to control the population and to develop temperature-sensitive mutants but there are some constrains that need to take into account.
The second limitation is related with the heat shock. This technique is commonly used to analyze behaviour and response to big changes in the environment but too much time exposure to high temperatures, always more than 25°C, can increase the production of males in the population . To avoid such a bias, it is important to tightly control the timing of temperature exposure.The first limitation for C. elegans populations is that if they are grown at more than 25°C the nematode can become sterile. The worms lose the capacity to reproduce; the majority of them are hermaphrodites and have the capacity to self-fertilize . Hence, there is a need to get an accurate temperature control when studying worm’s physiology.
Combining fast acting embryo mutants and live-cell imaging
Given the numerous aspects of C. elegans that are controlled by temperature changes, temperature controller systems can be crutial. Until 2014, looking at early onset protein response to fast temperature change was not possible due to the lack of adequate device. A 2016 publication compared the use of two systems allowing fast temperature changes while performing high resolution live cell imaging of embryos .
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