Yeast: Cell cycle and thermosensitive mutations


Temperature control for S. pombe cell cycle studies

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Working with S. pombe is working with one of the most powerful tools in genetics. You can scan all aspect of cell division, microtubule assembly, cell polarity and do phenotype-genotype analyses in a science minute! Experiments go faster in fission yeast, it is the beauty of this model. So how about going even faster in your research?.

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Schizosaccharomyces pombe

S. pombe is an african fission yeast isolated in 1893 from millet beer. It is a unicellular eukaryote organism with a caracteristic rode shape. S. pombe diameter is around 3 micrometers and depending on its cell cycle phase status, and a cell can measure between 8 and 13 micrometers. With a short generation time of 2 to 4 hours and straightforward genetics, S.pombe was at the origin of groundbreaking work on cell cycle regulation.[/vc_column_text][vc_column_text]

Temperature-control-microtubule-fission-yeast (3)

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Use of temperature-sensitive mutant in S. pombe fission yeast for live-cell imaging

[/vc_column_text][vc_column_text width=”1/1″ el_position=”first last”]During S. pombe division, the Cdc25 protein is required for mitotic entry.  In Cdc25 thermosensitive mutants,  the Cdc25 protein is functional at 25°C (Fig. 1a) but over 37°C it becomes non-functional or inactivated (Fig. 1b). Using Cdc25 t.s mutants allows for a reversible cell cycle arrest at the G2/M transition and a synchronous re-entry into the cell cycle upon shift to permisive temperature.

Fig.1: CDC25 protein is fonctional at 25°C (a) but becomes inactivated at 37°C (b)
Fig.1: Cdc25 protein is functional at 25°C (a) but becomes inactivated at 37°C (b)

[/vc_column_text][/vc_column][vc_column width=”1/1″][vc_text_separator title=”Reference” icon=”none” border=”yes_border” margin=”20″][vc_column_text]B. Alberts et al., Molecular Biology of the cell, 4th edition
P Nurse, P. Thuriaux, et al., Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe, Molec Gen Genet, 1976
M. Mitchison, The biology of the cell cycle. Cambridge University Press, 1971[/vc_column_text][/vc_column][vc_column width=”1/1″][vc_separator height_2=”20″ height=”20″ show_border=”no_border”][vc_text_separator title=”Scientitic Notes” icon=”krown-icon-briefcase” border=”yes_border” margin=”30″][vc_column_text]

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CherryTemp thermal stage

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Discover the CherryTemp Heater Cooler

Ultra-fast shifts and temperature accuracy at the sample level

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