AIMS
→ Human kidney culture for 7 days
→ Automatic perfusion at a flow rate
→ Live optical readouts acquisition (fluorescence)
FEATURES
→ Temperature : 37°C
→ Gas : normoxia, 5% CO2
→ Perfusion mode : pulse
→ Culture unit : 24 well multi well
RESULTS
→ Human kidney has been maintained over 7 days of culture
→ Live observation allows to follow glomerulus development
→ Histology preserved compared to previous protocol
PERSPECTIVE
→ Endocrine disruptors and nephrotoxic compounds testing and characterization
→ Extended to 3 weeks of culture for long term/chronic effect
