Nano4Stroke
Anti-inflammatory miRNA nanoshuttles as therapeutic strategy for stroke
Objectives
- Develop and select liposomes encapsulated miRNA with an activity on post stroke inflammation
- Develop a neuro vascular Unit (NVU) on chip to test the anti-inflammatory potential of the developed encapsulated miRNA
- Compare in vitro and in vivo data of the efficacy of anti-inflammatory miRNA
Our role
To generate an ischemic NVU-on-chip
model, WP2 will rely on the CubiX microenvironment controller developed by P2 and will entail the development of a new tailored culture unit based on the expertise of P4 on ischemic NVU.
Four steps will be needed:
- Development of ischemic NVU-on-chip prototype (specification sheet writing, designing, prototyping, quality control);
- Establishment of first protocol of use and hardware iterations;
- Refinement of the protocol;
- Biological validation of the culture unit.
Expected Results
- 1. Obtained an in vitro model for a functional healthy or ischemic Blood Brain Barrier.
- 2. Functionalize liposomes to be recruited by an inflamed (stroke mimicking condition) for a target delivery
- 3. Selecting of the best anti-inflammatory miRNA
Managing team
Pierre Gaudriault
Scientific Project Manager
Dario Fassini
Biological Model Validation Responsible
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Our expertise combines Microfluidic, 3DCell culture, Microscopy, Omics and artificial intelligence.
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