Mitosis control

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C. elegans mitosis control with thermosensitive mutants


An example using CherryTemp™ for fast and accurate temperature switch during microscopic observation.

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During C. elegans embryo division, the CYK-4 protein is required for the completion of cytokinesis (but not to form a furrow or initiate ingression). At the “permissive” 16°C temperature, the CYK-4 protein is functional but over 25°C it becomes inactivated. Such a mutant is called “thermosensitive”.

[/vc_column_text][vc_separator height_2=”30″ height=”40″ show_border=”no_border”][vc_text_separator title=”CherryTemp-induced cell division phenotype” icon=”none” border=”yes_border” margin=”17″ width=”1/1″ el_position=”first last”][vc_column_text width=”1/1″ el_position=”first last”]During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis blocking. Fig 1 shows unprecedently observed phenomenon during cell division.

CherryTemp offers unique properties to get the best tuning of C. elegans samples temperature and obtain results that could not be observed otherwise![/vc_column_text][/vc_column][vc_column width=”1/2″ el_position=”last”][vc_column_text]Cyk4

Figure 1.

C. elegansCYK-4thermosensitive mutant embryoswere shifted from 16°C to 25°C. Dividing embryosat 16°C (blue bar) underwent cytokinesis arrest after shift to restrictive temperature (25°C, CYK-4 inactivation) (red bar). White arrows indicate cleavage furrow ingression and regression respectively.
Courtesy of Julien Dumont (Dumont lab, Institut Jacques Monod, France)

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Emulating human cell & tissue physiology

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