What is the organotypic culture ?
Organotypic culture is defined as the culture of an organ collected from an organism. It is one method allowing the culture of complex tissues or organs. It allows the preservation of the architecture of the cultured organ and most of its cellular interactions.
Figure 1: Illustration of various organotypic culture method.
Several protocols have already been developed like agar methods, watch glass protocols, organ culture in media, and filter well inserts.
While cells lines are one of the most often used experimental model by scientists, surprisingly, culture of whole organs start few decades before the first establishment of cell lines.
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Written by Pierre Gaudriault
Innovation Unit | Intrapreneur
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