Protocol For Drosophila Optic Lobes

Mounting method for D. Melanogaster optic lobes

This protocol is for short term imaging (up to 2 hours) of optic lobes. Spacers can replace standard methods which use glass coverslips, glycerol and halocarbon oil drops as mounting materials. For long-term imaging protocols, spacers can be replaced by gas permeable materials.

Optic lobes of larva, pupa and adult can be imaged.

Dissection of brains can be performed using standard protocols. 5 to 10 brains can be used for imaging.

Step-by-step guidelines:

Step 1: Top a glass coverslip with our dedicated spacer (spacer for optic lobes: 200µM thickness: equivalent to the thickness of a coverslip).

Step 2: Place a drop of 5ul of mounting medium and use forceps to place the brains. Place the medium in the spacer’s hole, without touching the sides of the spacer. Place the brains with the wanted orientation.

Step 3: Top the spacer with the CherryTemp chip. Place the chip with the correct orientation (thermalisation chamber facing down, in contact with the spacer). There is no need of further sealing.

Step 4: Place the mounting system on either an upright or an inverted configuration depending on the microscope setup.



Drosophila Neurobiology Manual, Copyright 2010. Cold Spring Harbor Laboratory Press (Chapter 10, Morante J., Desplan, C.)

Related Posts

Microtubule Dynamics And MAPs...
Introduction Microtubules are polymers of tubulin involved in multiple cellular processes, including mitotic spindle formation, cell transp...
Read more
Protocol for Drosophila larval brains...
Mounting method for third larval brains of D. Melanogaster Introduction This protocol shows how to mount Drosophila 3d instar larval brains ...
Read more
Fruit Fly Pupae...
Introduction Drosophila pupae have been used in research as a model to study developmental transitions like metamorphosis. The developmental...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!