Publications

Fast Temperature Controller For Live-cell Imaging Cytoskeleton Studies

Summary

Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment. One such control is the ability to quickly change the temperature of the cells. Genetic model organism such as fission yeast has been useful for studies of the cell cytoskeleton. In particular, the dynamic microtubule cytoskeleton responds to changes in temperature. In addition, there are temperature-sensitive mutations of cytoskeletal proteins. We describe here the fabrication and use of a microfluidic device to quickly and reversibly change cellular temperature between 2°C and 50°C. We demonstrate the use of this device while imaging at high-resolution microtubule dynamics in fission yeast.

Publication source

Related Posts

Yeast division
An easy-to-build and re-usable microfluidic system for live-cell ...
Background Real-time monitoring of cellular responses to dynamic changes in their environment or to specific treatments has become central t...
Read more
Yeast: Spindle And Microtubule Assembly...
Temperature control for spindle assembly studies in fission yeast S. pombe is a very powerful organism to study microtubules assembly. The e...
Read more
Therma Flow: Lectin Protocol For Yeast...
Introduction The following protocol explains how to use the temperature controller CherryTemp with the Therma Flow chip for medium perfusion...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!