Application notes

Fission Yeast Cells: Temperature Shifts From 5°C To 30°C

CherryTemp & the Zeiss Definite Focus 2

The Zeiss France team co-wrote a document together with the teams of Damien Coudreuse (IGDR, Rennes) and Jenny Wu (IGDR, Rennes). This note entitled “Hardware Autofocus enables Long-term Imaging of Cells Grown in Microfluidic devices” focuses on the use of cutting-edge microfluidic devices installed on a Zeiss Axio Observer (Carl Zeiss Inc.) equipped with a Definite Focus 2 module. The CherryTemp system (CherryBiotech) is used to thermalize the yeast strains and perform fast shifts and a precise OB1 pressure generator (Elveflow) allows fast medium changes and stable actuation through home made microfluidic chips.

The follow video is co-published with Zeiss. It shows how the Definite Focus 2 module is able to compensate an impressive 50µm loss-of focus induced by temperature dilatation.

Fission yeast cells were subjected to temperature shifts from 5°C to 30°C.

Loss of focus occurs as a consequence of dilatation due to temperature changes.

This video shows how the Zeiss Definite Focus 2 module is able to compensate a 50µm loss-of focus. Samples were imaged on a Zeiss LSM 800 microscope.

Video co-published between Cherry Biotech and Zeiss France during the Yeast Symposium in Toulouse 2016.

Ultra fast temperature shift device for in vitro experiments under microscopy​

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