Publications

Fast Microfluidic Temperature Control During High Resolution Live Cell Imaging

Summary

One major advantage of using genetically tractable model organisms such as the fission yeastSchizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature-sensitive mutation enables precise temporal control ofprotein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 °C and 45 °C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle.

Publication source

Related Posts

Fission Yeast Cells: Temperature Shifts From 5°C To 30°C...
CherryTemp & the Zeiss Definite Focus 2 The Zeiss France team co-wrote a document together with the teams of Damien Coudreuse (IGDR, Re...
Read more
Controlling Lifetimes Of Exocytic Events With Fast Shift Temperat...
Yeast cells strains expressing a GFP labeled exocyst complex component  were grown overnight in standard yeast peptone dextrose medium (YPD...
Read more
Fast Microfluidic Temperature Control During High Resolution Live...
Summary One major advantage of using genetically tractable model organisms such as the fission yeastSchizosaccharomyces pombe is the abilit...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!