Application notes

Yeast Cold-shock Microtubule Depolymerization

Experimental conditions

S. pombe microtubules were depolymerized by cold shock by shifting cells to 5°C. Fission yeast cells were grown at 25°C and placed on a CherryTemp mounting system for live imaging. Cells were shifted from 25°C to 5°C in less than 10 sec, inducing microtubule depolymerization. Microtubulerepolymerization occurred upon shifting back the temperature to 25°C.

Cells were imaged on a Nikon Ti Eclipse spinning disk. Images were acquired every 20 seconds. Microtubules were visualized withgamma-tubulin GFP

Ultra fast temperature shift device for in vitro experiments under microscopy​

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