Application notes

Yeast Cold-shock Microtubule Depolymerization

Experimental conditions

S. pombe microtubules were depolymerized by cold shock by shifting cells to 5°C. Fission yeast cells were grown at 25°C and placed on a CherryTemp mounting system for live imaging. Cells were shifted from 25°C to 5°C in less than 10 sec, inducing microtubule depolymerization. Microtubulerepolymerization occurred upon shifting back the temperature to 25°C.

Cells were imaged on a Nikon Ti Eclipse spinning disk. Images were acquired every 20 seconds. Microtubules were visualized withgamma-tubulin GFP

Ultra fast temperature shift device for in vitro experiments under microscopy​

Related Posts

Therma Flow: Lectin Protocol For Yeast...
Introduction The following protocol explains how to use the temperature controller CherryTemp with the Therma Flow chip for medium perfusion...
Read more
Yeast: Spindle And Microtubule Assembly...
Temperature control for spindle assembly studies in fission yeast S. pombe is a very powerful organism to study microtubules assembly. The e...
Read more
Fast Temperature Controller For Live-cell Imaging Cytoskeleton St...
Summary Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!