Application notes

Yeast Cold-shock Microtubule Depolymerization

Experimental conditions

S. pombe microtubules were depolymerized by cold shock by shifting cells to 5°C. Fission yeast cells were grown at 25°C and placed on a CherryTemp mounting system for live imaging. Cells were shifted from 25°C to 5°C in less than 10 sec, inducing microtubule depolymerization. Microtubulerepolymerization occurred upon shifting back the temperature to 25°C.

Cells were imaged on a Nikon Ti Eclipse spinning disk. Images were acquired every 20 seconds. Microtubules were visualized withgamma-tubulin GFP

Ultra fast temperature shift device for in vitro experiments under microscopy​

Related Posts

Agarose Pad Protocol For Yeast...
Introduction For full efficiency of the CherryTemp temperature controller, samples must be placed close to the CherryTemp thermalisation chi...
Read more
Fission Yeast Cells: Temperature Shifts From 5°C To 30°C...
CherryTemp & the Zeiss Definite Focus 2 The Zeiss France team co-wrote a document together with the teams of Damien Coudreuse (IGDR, Ren...
Read more
S. Pombe : Temperature Control Of Microtubules Assembly...
Temperature control of microtubules assembly in S. pombe Abstract S. pombe is a very powerful organism to study microtubules assembly. The ...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!
As part of our commercial prospecting, we may need to process your personal data. For more information, please consult our Privacy Policy