Application notes

Yeast cold-shock microtubule depolymerization

Experimental conditions

S. pombe microtubules were depolymerized by cold shock by shifting cells to 5°C. Fission yeast cells were grown at 25°C and placed on a CherryTemp mounting system for live imaging. Cells were shifted from 25°C to 5°C in less than 10 sec, inducing microtubule depolymerization. Microtubulerepolymerization occurred upon shifting back the temperature to 25°C.

Cells were imaged on a Nikon Ti Eclipse spinning disk. Images were acquired every 20 seconds. Microtubules were visualized withgamma-tubulin GFP

Discover CherryTemp - Ultra-fast temperature shift for live cell imaging under microscopy

Related Posts

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!
Scroll to Top