Protocols

Lectin protocol for yeast

Concanavalin A mounting protocol

Introduction

The following protocol explains how to use the temperature controller CherryTemp when yeast cells are mounted on lectin coated slides. Yeast cells are deposited on lectin coated coverslips and our dedicated spacers allow to simply place the CherryTemp chip on top. Lectin helps to reduce cell movement for optimal imaging and CherryTemp allows for an accurate temperature control without disturbing cells.

No shear stress and an easy protocol for rapid mounting and imaging of yeasts.

Step-by-step guidelines:

Step 1: Pipet a drop of lectin (Concanavalin A, Con A) on a high-resolution glass coverslip.

Lectin-protocol-for-yeast_Fig-01-e1530709626406

Step 2: Let the liquid evaporate under the hood.

Step 3: Top the coverslip with our dedicated spacer.

Lectin-protocol-for-yeast_Fig-03-e1530716480764

Step 4: Drop the yeast sample (up to 15µl) centred in the spacer hole. Concentration of cells is recommended to achieve an optimal cell density.

 
Lectin-protocol-for-yeast_Fig-04-e1530707628958

Step 5:

Top with the CherryTemp chip (engraved arrows facing up, glass surface and microfluidic chamber facing the sample).

Lectin-protocol-for-yeast_Fig-05-e1530707637295

Step 6:

Place the mounted slide on either an upright or an inverted configuration by simply flipping it.

Lectin-protocol-for-yeast_Fig-06-e1530707666463

References

Methods adapted from P. Tran lab (Institut Curie) and from our user’s feedback.

Related Posts

Cold Induced Microtubule Depolymerization In Fission Yeast...
Abstract We developed a temperature controller that meets cell biology studies requirements. It reaches below ambient temperatures for effic...
Read more
S. Pombe : Temperature Control Of Microtubules Assembly...
Temperature control of microtubules assembly in S. pombe Abstract S. pombe is a very powerful organism to study microtubules assembly. The ...
Read more
Lectin-protocol-for-yeast_Fig-06-e1530707666463
Lectin protocol for yeast...
Concanavalin A mounting protocol Introduction The following protocol explains how to use the temperature controller CherryTemp when yeast ce...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!