Scientific notes

Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos

Through chromosomes tracking during C. elegans embryo, news findings on their conformation and the function of lamina in chromosomes stretching

To better understand chromosome segregation Ahilya N. Sawh et al. worked on Caenorhabditis elegans embryogenesis and developed a chromosome tracking method. They notice an early barbell-like organization showing that not all epigenetically similar domains were consolidated in space. This organization come from lamina tethering which overrides homotypic B-compartment interactions in early C. elegans embryo. They conclude that tethering to the lamina stretches chromosomes over any genomic distances allowing the barbell organization. These findings highlight a new main role of the lamina and also new conformations during early embryogenesis.

Whole-chromosome tracing in developing C. Elegans Embryos [digital image].

Ultra fast temperature shift device for in vitro experiments under microscopy


Current models suggest that chromosome domains segregate into either an active (A) or inactive (B) compartment. B-compartment chromatin is physically separated from the A compartment and compacted by the nuclear lamina. To examine these models in the developmental context of C. elegans embryogenesis, we undertook chromosome tracing to map the trajectories of entire autosomes. Early embryonic chromosomes organized into an unconventional barbell-like configuration, with two densely folded B compartments separated by a central A compartment. Upon gastrulation, this conformation matured into conventional A/B compartments. We used unsupervised clustering to uncover subpopulations with differing folding properties and variable positioning of compartment boundaries. These conformations relied on tethering to the lamina to stretch the chromosome; detachment from the lamina compacted, and allowed intermingling between, A/B compartments. These findings reveal the diverse conformations of early embryonic chromosomes and uncover a previously unappreciated role for the lamina in systemic chromosome stretching.


Related Posts

Exploratory Analysis Of Transposable Elements Expression In The C...
Introduction Transposable Elements are expressed in the C. elegans early embryo and involved in his development, but they might also have ...
Read more
Protocol For C. elegans Embryos With Spacers...
Use our dedicated spacers to mount C. elegans temperature-sensitive embryos Introduction This protocol shows how to mount C. elegans embry...
Read more
The Daf-2 Insulin Receptor Functions In C. elegans Embryo Elongat...
Role of Daf-2 insulin signaling in the embryonic elongation of C. elegans, a daf-2 loss of function seems to vary according to the allele in...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!