C.elegans thermosensitive mutants
During C. elegans embryo division, the CYK-4 protein is required for the completion of cytokinesis (but not to form a furrow or initiate ingression). At the “permissive” 16°C temperature, the CYK-4 protein is functional but over 25°C it becomes inactivated. Such a mutant is called “thermosensitive”.
Ultra fast temperature shift device for in vitro experiments under microscopy
CherryTemp-induced cell division phenotype
During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis blocking. Fig 1 shows unprecedently observed phenomenon during cell division.
CherryTemp offers unique properties to get the best tuning of C. elegans samples temperature and obtain results that could not be observed otherwise!