C. elegans mitosis control with thermosensitive mutants
An example using CherryTemp™ for fast and accurate temperature switch during microscopic observation.
C.elegans thermosensitive mutants
During C. elegans embryo division, the CYK-4 protein is required for the completion of cytokinesis (but not to form a furrow or initiate ingression). At the “permissive” 16°C temperature, the CYK-4 protein is functional but over 25°C it becomes inactivated. Such a mutant is called “thermosensitive”.
CherryTemp-induced cell division phenotype
During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis blocking. Fig 1 shows unprecedently observed phenomenon during cell division.
CherryTemp offers unique properties to get the best tuning of C. elegans samples temperature and obtain results that could not be observed otherwise!
Ultra fast temperature shift device for in vitro experiments under microscopy
References
Courtesy of Julien Dumont (Dumont lab, Institut Jacques Monod, France)
