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Mitosis control

C. elegans mitosis control with thermosensitive mutants

An example using CherryTemp™ for fast and accurate temperature switch during microscopic observation.

C.elegans thermosensitive mutants

During C. elegans embryo division, the CYK-4 protein is required for the completion of cytokinesis (but not to form a furrow or initiate ingression). At the “permissive” 16°C temperature, the CYK-4 protein is functional but over 25°C it becomes inactivated. Such a mutant is called “thermosensitive”.

CherryTemp-induced cell division phenotype

During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis blocking. Fig 1 shows unprecedently observed phenomenon during cell division.

CherryTemp offers unique properties to get the best tuning of C. elegans samples temperature and obtain results that could not be observed otherwise!

Fig 1: CYK-4 ts C. elegans control of cytokinesis by CherryTemp When temperature is set to 16°C, cell division goes on normally (a-f). Immediately after quick change of temperature (25°C) the CYC-4 protein is inactivated resulting in cytokinesis arrest.

Fig 1: CYK-4 ts C. elegans control of cytokinesis by CherryTemp
When temperature is set to 16°C, cell division goes on normally (a-f). Immediately after quick change of temperature (25°C) the CYK-4 protein is inactivated resulting in cytokinesis arrest.


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