Exocytosis & phase separation related contents
Among cellular mechanisms which can be controlled by temperature, protein movement based ones are of greatest interest. The general topic of vesicular trafficking is a perfect example of such temp dependent event. Exocytosis, endocytosis, protein association at the plasma membrane…all are highly dynamic processes which can be tightly controlled by temperature.
A second category of mechanisms concerns protein aggregation and the so-called “phase separation” processes. An illustration is given (Cell 2018) focusing on the FUS proteins and their association and disassociation in the cytoplasmic or nuclear compartments.
Controlling lifetimes of exocytic events
Yeast cells strains expressing a GFP labeled exocyst complex component were grown overnight in standard yeast peptone dextrose medium (YPD) and were cultured at 30°C with continuous shaking at 280 rpm. After overnight culture, cells were washed 3x with H2O and diluted 10-fold in SC-All media for 2h at 30°C…. READ FULL CONTENT
CherryTemp thermalising unit
Nuclear import receptor inhibits phase separation of FUS through binding to multiple sites
Cell compartmentalization is essential for cellular homeostasis. Cytoplasmic compartments can be delineated by molecular concentrates, generated by the physical mechanism of liquid-liquid phase separation (LLPS). One example of compartments generated by molecular condensation is the one involving the protein FUS…READ FULL CONTENT
Liquid Phase Separation
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