Scientific notes

Requirement For Translocon-associated Protein (TRAP) Α In Insulin Biogenesis

Engineered C. elegans as a model organism for a better understanding of type 2 diabetes in humans

To better understand the mechanisms of peptide hormones, Xin Li et al. performed a research on translocon associated protein α (TRAPα). They reveal a primordial role of TRAPα in the biosynthesis of insulin. They have used C. elegans as a model organism for mammals to better understand the pathogenesis of type 2 diabetes. The insulin secretion pathway analysis demonstrates the importance of the endoplasmic reticulum translocation machinery in insulin biogenesis. To further extend these findings in rats and humans, they experimented in pancreatic β cells of rats where TRAPα would directly promote preproinsulin translocation, influences proinsulin maturation & insulin secretion. All these advances conduct to suggestions that preproinsulin translocation and proinsulin trafficking could lead to the development of type 2 diabetes.

Spatiotemporal expression of a functional TRAP-1::mCherry fusion protein. [digital image].

Ultra fast temperature shift device for in vitro experiments under microscopy

Abstract

“The mechanistic basis for the biogenesis of peptide hormones and growth factors is poorly understood. Here, we show that the conserved endoplasmic reticulum membrane translocon-associated protein α (TRAPα), also known as signal sequence receptor 1, plays a critical role in the biosynthesis of insulin. Genetic analysis in the nematode Caenorhabditis elegans and biochemical studies in pancreatic β cells reveal that TRAPα deletion impairs preproinsulin translocation while unexpectedly disrupting distal steps in insulin biogenesis including proinsulin processing and secretion. The association of common intronic single-nucleotide variants in the human TRAPα gene with susceptibility to type 2 diabetes and pancreatic β cell dysfunction suggests that impairment of preproinsulin translocation and proinsulin trafficking may contribute to the pathogenesis of type 2 diabetes.”

References

Related Posts

Using the Four-Cell C. elegans Embryo to Study Contractile Ring D...
Discover new in vivo protocols to understand contractile ring dynamics during cytokinesis in C. elegans embryo Fung Yi Chan, Ana Marta Silva...
Read more
Sydney Brenner
Tribute to C. elegans Nobel Prize : Sydney Brenner...
THE GENETICS OF CAENORHABDITIS ELEGANS (1974) Sydney Brenner shed light on the Caenorhabditis elegans in 1974 with his study demonstrating...
Read more
C.elegans worm
Centrosome Aurora A Regulates RhoGEF ECT-2 Localisation And Ensur...
Introduction Research has already shown a proper establishment of cell polarity is vital for several events during development stages and mo...
Read more

get in touch

Get the best insights about Cherry Biotech by Email Let’s stay in touch!
As part of our commercial prospecting, we may need to process your personal data. For more information, please consult our Privacy Policy