CherryTemp™Heater/Cooler stage for C. elegans

ULTRAFAST : 10 SECONDS TEMPERATURE SHIFTS

Cell division, ts mutants or cytoskeleton studies

LARGE RANGE : FROM 5°C TO 45°C

High precision – unprecedented reproducibility

FOLLOW TS MUTANTS WITH ACCURACY

Embryos or adult worm studies.

CherryTemp fits a wide range of high resolution microscopes.

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Heat and cool down your samples in the 5 to 45°C range

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mCherry-tag C. elegans thermosensitive embryo mutants (CYK-4 ts) examined under the microscope with a CherryTemp platform.

EASY EMBRYO DISSECTION :

“CherryTemp allows us to perform temperature shift experiments with unprecedented speed and precision with C. elegans embryos. It is very well designed and easy to use. Moreover, we found the the customer service at Cherry Biotech to be very responsive and competent.”
Visit the Gönczy lab.

Prof. Pierre Gönczy

EPFL, SWITZERLAND

Methods & Scientific notes

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STABLE & PRECISE | 0.1°C :

“The Cherry system is now in full use in my lab and it is working beautifully on its own.
The Cherry system is great for stably maintaining temperature on our microscopes”

Visit the Canman lab 

Dr. Julie Canman

COLUMBIA UNIVERSITY MEDICAL CENTER

Easy mounting of your worm samples

temperature-heater-cooler-for-c-elegans-embryos-live-cell-imaging2

Mount your worms / embryos easily using our set-up.

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FITS HIGH-RESOLUTION MICROSCOPES (UPRIGHT & INVERTED):

“The Cherry Temp is perfect for use in imaging core facilities. Its design allows its installation on a wide panel of upright and inverted microscopes and easy training of users thus, promoting development of innovating research project. The customer service is always helpful and responsive.”
Visit Dr Dutertre’s core facility MRic

Stéphanie Dutertre, Instrument core facility manager

BIOSIT LABORATORY, CNRS 3480 - INSERM 018, MRic

Our videos

ULTRAFAST SHIFTS | 10 SECONDS :

“CherryTemp rapidly became one of our favorite piece of equipment in the lab.
It’s easy to use and works fast and well. The Cherry Biotech team is always super helpful.”

Visit Julien Dumont’s Lab

Dr. Julien Dumont

INSTITUT JACQUES MONOD, FRANCE

Publications

C. elegans chromosome segregation: microtubule pushing

C. elegans chromosome segregation: microtubule pushing

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Inhibition of microtubule assembly using fast temperature changes

Inhibition of microtubule assembly using fast temperature changes

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Medium renewal and temperature control for live-cell imaging

Medium renewal and temperature control for live-cell imaging

(Open Biology, 2016) Summary | Monitoring cellular responses to changes in growth conditions and perturbation of targeted pathways is integral to...
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Real-time qPCR using CherryTemp on-a-chip

Real-time qPCR using CherryTemp on-a-chip

(Lab on Chip, 2016) Summary | To control future infectious disease outbreaks, like the 2014 Ebola epidemic, it is necessary to...
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GET A QUOTE OR TECHNICAL INFORMATION

Heat and cool down your samples in the 5 to 45°C range

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Application example: C. elegans embryogenesis control by CherryTemp

During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis arrest. This outlined unprecedently observed phenomenon during cell division.

temperature-heater-cooler-for-microscopy-c-elegans-embryo-cyk4-ts-mutant

Timelapse images of the 1st division of C. elegans embryo movie:

Divisions were recorded using a microscope (DM6000; Leica) equipped with epifluorescence and DIC optics and a camera (DFC 360 FX; Leica). Images were collected every 15 sec using a 63×/1.4 NA objective and LAS AF software (Leica). Embryos were imaged at 22°C and the temperature was shifted to 5°C at 0sec thanks to the special control stage equipment. After 195 sec at 5°C,  the temperature was shifted back to 22°C. Temperature control and tempertaure shifts were performed using Cherry Temp. more information about this application note

Images representative of timelapse images of the 1st division of C. elegans embryo

Benefit from a smart platform

No shear stress

Our thermalization technology does not interfere with your cells. They remain happy in a standard two-cover-slips sandwich.

Long-term stability

Thanks to our spacer, you can add medium and avoid evaporation. In addition, we have a closed fluidic circuit for a long-term and stable thermalization of your sample;

First installation in 20 minutes

You want to focus on your experimentation this is why we’ve made CherryTemp easy to install. CherryTemp is plug and play and comes along with a didactic installation guide.

No interference with your set-up

Your microscope set-up can already be complex this is why we’ve designed CherryTemp to ensure a smooth integration.

GET A QUOTE OR TECHNICAL INFORMATION

Heat and cool down your samples in the 5 to 45°C range

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Access to the amazing worm community!

Do not miss any worm meeting: http://wormmeetings.weebly.com/

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