Dedicated Pack for C. Elegans

Temperature control of C. elegans samples during live-cell imaging

We developped the world’s fastest heater-cooler for microscopy.
It enables ultra-fast temperature shifts [10 seconds] from any temperature to any other in the 5-45°C range.

CherryTemp fits uprights ad inverted microscopes.
Now you can image and control the temperature  of your favorite C.elegans.


Induce entry or exit of “Dauer” stage


Heat shock: control the timing of temperature exposure


Used for embryos or adult worm studies.


Follow TS mutants with accuracy


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Heat and cool down your samples in the 5 to 45°C range

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mCherry-tag C. elegans thermosensitive embryo mutants (CYK-4 ts) examined under the microscope with a CherryTemp platform.

EASY EMBRYO DISSECTION :

“Cherry Temp allows us to perform temperature shift experiments with unprecedented speed and precision with C. elegans embryos. It is very well designed and easy to use. Moreover, we found the the customer service at Cherry Biotech to be very responsive and competent.”
Visit the Gönczy lab.

Prof. Pierre Gönczy

EPFL, SWITZERLAND

Methods & Scientific notes

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Scientifique note for C. elegans

View all applications →

STABLE & PRECISE | 0.1°C :

“The Cherry system is now in full use in my lab and it is working beautifully on its own.
The Cherry system is great for stably maintaining temperature on our microscopes”

Visit the Canman Lab 

Dr. Julie Canman

COLUMBIA UNIVERSITY MEDICAL CENTER

Easy mounting of your worm samples

temperature-heater-cooler-for-c-elegans-embryos-live-cell-imaging2

Mount your worms / embryos easily using our set-up.

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FITS BOTH INVERTED & UPRIGHT MICROSCOPES :

“The Cherry Temp is perfect for use in imaging core facilities. Its design allows its installation on a wide panel of upright and inverted microscopes and easy training of users thus, promoting development of innovating research project. The customer service is always helpful and responsive.”
Visit Stephanie Dutertre core facility MRic

Stéphanie Dutertre, Instrument core facility manager

BIOSIT LABORATORY, CNRS 3480 - INSERM 018, MRic

Our videos

ULTRAFAST SHIFTS | 10 SECONDS :

“CherryTemp rapidly became one of our favorite piece of equipment in the lab.
It’s easy to use and works fast and well. The Cherrybiotech team is always super helpful.”

Visit Julien DUMONT ‘s Lab

Dr. Julien Dumont

INSTITUT JACQUES MONOD, FRANCE

Publications

Inhibition of microtubule assembly using fast temperature changes

Inhibition of microtubule assembly using fast temperature changes

(Development, 2017) Summary | In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and chromosome segregation – essential to prevent...
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Medium renewal and temperature control for live-cell imaging

Medium renewal and temperature control for live-cell imaging

(Open Biology, 2016) Summary | Monitoring cellular responses to changes in growth conditions and perturbation of targeted pathways is integral to...
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Real-time qPCR using CherryTemp on-a-chip

Real-time qPCR using CherryTemp on-a-chip

(Lab on Chip, 2016) Summary | To control future infectious disease outbreaks, like the 2014 Ebola epidemic, it is necessary to...
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Temperature control of thermosensitive protein regulation

Temperature control of thermosensitive protein regulation

(Developmental cell, 2009) Summary | The spindle midzone—composed of antiparallel microtubules, microtubule-associated proteins (MAPs), and motors—is the structure responsible for microtubule...
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GET A QUOTE OR TECHNICAL INFORMATION

Heat and cool down your samples in the 5 to 45°C range

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Application example: C. elegans embryogenesis control by CherryTemp

During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis arrest. This outlined unprecedently observed phenomenon during cell division.

temperature-heater-cooler-for-microscopy-c-elegans-embryo-cyk4-ts-mutant

Timelapse images of the 1st division of C. elegans embryo movie:

Divisions were recorded using a microscope (DM6000; Leica) equipped with epifluorescence and DIC optics and a camera (DFC 360 FX; Leica). Images were collected every 15 sec using a 63×/1.4 NA objective and LAS AF software (Leica). Embryos were imaged at 22°C and the temperature was shifted to 5°C at 0sec thanks to the special control stage equipment. After 195 sec at 5°C,  the temperature was shifted back to 22°C. Temperature control and tempertaure shifts were performed using Cherry Temp. more information about this application note

Images representative of timelapse images of the 1st division of C. elegans embryo

Benefit from a smart platform

No shear stress

Our thermalization technology does not interfere with your cells. They remain happy in a standard two-cover-slips sandwich.

Long-term imaging

Thanks to our spacer, you can add medium and avoid evaporation. In addition, we have a closed fluidic circuit for a long-term and stable thermalization of your sample;

First installation in 20 minutes

You want to focus on your experimentation this is why we’ve made CherryTemp easy to install. CherryTemp is plug and play and comes along with a didactic installation guide.

No interference with your set-up

Your microscope set-up can already be complex this is why we’ve designed CherryTemp to ensure a smooth integration.

GET A QUOTE OR TECHNICAL INFORMATION

Heat and cool down your samples in the 5 to 45°C range

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