Control temperature of C. elegans samples:  C. elegans Pack

We developped the world’s fastest heater-cooler for microscopy. It enables ultra-fast temperature shifts [10 seconds] from any temperature to any other in the 5-45°C range. CherryTemp fits uprights ad inverted microscopes. You can now control in real-time your favorite molecular processes, observe dynamic events linked to temperature on live and discover unthought mechanisms.

Cherry Temp allows us to perform temperature shift experiments with unprecedented speed and precision with C. elegans embryos. It is very well designed and easy to use. Moreover, we found the the customer service at Cherry Biotech to be very responsive and competent.

Visit the Gönczy lab.

Prof. Pierre Gönczy

GÖNCZY LAB, EPFL, SWITZERLAND

Application example: C. elegans embryogenesis control by CherryTemp

During our experimentation, C. elegans thermosensitive embryo mutants (CYK-4 ts) were examined under the microscope with a CherryTemp platform. This system allowed to selectively modify the temperature of embryo samples between 16°C and 25°C resulting in cytokinesis arrest. This outlined unprecedently observed phenomenon during cell division.

temperature-heater-cooler-for-microscopy-c-elegans-embryo-cyk4-ts-mutant


C. elegans imaging & temperature

BELOW AMBIANT | 5 TO 45°C

Play with ts mutants from 15 to 26°C and more!

EASY EMBRYO DISSECTION

Directly dissect and place embryos on our dedicated device!

ULTRAFAST SHIFTS | 10 SECONDS

Shift in less than 10 seconds and observe restrictive temperatures on live

STABLE & PRECISE | 0.1°C

Fine-tune permissive and restrictive temperatures

FITS ANY MICROSCOPE

CherryTemp can be easily installed and shared between different microscopes. We have a set of ergonomic elements fitting main brands (Nikon, Zeiss, Olympus, Leica) for both upright and inverted scopes.

Section

From worm dissection to observation in 5 minutes

Change the temperature of your sample within seconds during live-cell imaging

Initially developed for PCR military application, the world fastest temperature controller for microscopy is now available for live-cell imaging.

Get benefit of our robust and precise temperature calibration. Our software continuously integrates changes of your room temperature. It also takes into account the heat sink played by immersion objective lenses.

Discover the C. elegans mounting specifications

Traditionally, C. elegans embryos have been imaged on standard microscope slides with a 2% agarose pad and a coverslip (Fig. 5A). This has the advantage of being easy to assemble and requiring no special equipment. Moreover, it is possibled with the use of an eyelash tool or ultrafine deer hairdto group embryos together, allowing simultaneous imaging of multiple embryos. Additionally, the process of sandwiching the embryo between the coverslip and the agarose pad results in a slight compression of the embryos, which can improve imaging of fluorescently tagged proteins.

An alternate method termed “hanging drop” traps the embryos in a drop of M9 buffer held between the slide and coverslip with a Vaseline petroleum jelly spacer (Fig. 5B). This prevents compression and results in the embryos being more randomly oriented relative to the plane of the slide. This inconsistency in orientation ensures, at least in a subset of the embryos, that imaging occurs at the optimum viewing angle for most cells during embryonic development

Benefit from the C. elegans Pack

No shear stress

Our thermalization technology does not interfere with your cells. They remain happy in a standard two-cover-slips sandwich.

Long-term imaging

Thanks to our spacer, you can add medium and avoid evaporation. In addition, we have a closed fluidic circuit for a long-term and stable thermalization of your sample.

Ready in 30 seconds

This mounting system is plug-and-play and respects microscopes standards. Just focus on your experimentation!

Benefit from a smart platform

No interference with your set-up

Your microscope set-up can already be complex this is why we’ve designed CherryTemp to ensure a smooth integration.

Fits any inverted microscope

You might want to use different microscopes, this is why we’ve designed CherryTemp to be compatible with any inverted microscope.

First installation in 20 minutes

You want to focus on your experimentation this is why we’ve made CherryTemp easy to install. CherryTemp is plug and play and comes along with a didactic installation guide.

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